摘要
目的:探索HPLC测定舒胸脉冲控释滴丸中阿魏酸和羟基红花黄色素A的含量。方法:采用Kromasil C18色谱柱(4.6 mm×250 mm,5μm),以甲醇-0.01 mol.L-1磷酸二氢钾水溶液(22∶78)用磷酸调节pH 3.0为流动相,流速1.0 mL.min-1,检测波长403,322 nm,柱温30℃。结果:阿魏酸在1.409～90.16 mg.L-1回归方程为A=0.820 6 C-0.287 4(r=0.9999),平均回收率为99.43%,RSD 3.42%(n=6);羟基红花黄色素A在4.035～258.3 mg.L-1,回归方程为A=0.412 7C-0.487 8(r=0.999 7),平均回收率为100.03%,RSD 3.55%(n=6)。结论:该方法操作简便,准确,重复性良好,可用于舒胸脉冲控释滴丸的质量控制。
Objective： To develop a HPLC method for determination of the content of ferulic acid and hydroxy safflower yellow A in Shuxiong pulsatile controlled-release dropping pill. Method： Kromasil C18 （4.6 mm×250 mm, 5 μm） column was used, methanol-0.01 molL-1 potassium dihydrogen phosphate （22： 78, pH was adjusted to 3.0 by phosphoric acid） as mobile phase. The flow rate was 1.0 mLmin-1, detection wavelength at 403, 322 nm, and column temperature at 30 ℃. Result： The linear response ranges were 1.409-90.16 mgL-1 with regression curve A=0.820 6 C-0.287 4 （r=0.999 9） for ferulic acid, 4.035-258.3 mgL-1 with regression curve A=0.412 7C-0.487 8 （r=0.999 7） for hydroxy safflower yellow A. The average recovery of ferulic acid and hydroxy safflower yellow A were 99.43% and 100.03% （RSD 3.42%, 3.55%, n=6）. Conclusion： A simple, accurate and reproducible HPLC method is provided for determination of ferulic acid and hydroxy safflower yellow A in Shuxiong pulsatile controlled-release dropping pill.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第10期92-94,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省自然科学基金项目(S2011010002485)
关键词
舒胸脉冲控释滴丸
高效液相色谱
阿魏酸
羟基红花黄色素A
含量测定
Shuxiong pulsatile controlled-release dropping pill
HPLC
ferulic acid
hydroxy safflower yellow A
content determination
