摘要
目的观察T-bet在胃癌组织中的表达及对患者的无病生存期、总生存期的影响。方法应用免疫组织化学染色技术，检测152例胃癌患者手术标本中T-bet阳性淋巴细胞的浸润数量，观察患者总生存期和无病生存期。结果T-bet阳性淋巴细胞的浸润数量与肿瘤大小（t=2．35，P〈0．05）、浸润深度（t=2．50，P〈0．05）、UICC分期（t=3．40，P〈0．05）有关，差异有统计学意义。所有患者中位无病生存期为28个月，中位总生存期为37个月。Kaplan—Meier生存分析结果表明，T-bet高表达组无病生存期（χ^2=3．91，P〈0．05）及总生存期（χ^2=4．00，P〈0．05）均优于低表达组，差异有统计学意义。COX多因素分析显示，调整年龄、性别、肿瘤部位、浸润深度、肿瘤大小和UICC分期后，T-bet高表达组胃癌患者的复发[风险比（HR）=0．56，95％可信区间（95％CI）=0．32—0．98，P〈0．05]及死亡风险降低（HR=0．55，95％CI=0．30～0．99，P〈0．05），差异有统计学意义（P〈0．05）。结论T—bet阳性淋巴细胞的浸润数量与胃癌患者的生存时间呈正相关，证实了T—bet为1个正性调节因子，对肿瘤微环境的免疫调节有重要意义。
Objective To analyze the expression of T-bet in gastric carcinoma tissues and the impact on the survival time of patients. Methods The number of T-bet positive lymphocyte in the surgical specimens of gastric cancer tissues was detected by using immunohistochemistry in 152 patients. The time of disease-free survival and overall survival were observed. Data was analyzed by using SPSS 13.0. Results The T-bet expression level was significantly correlated with tumor size （ t = 2. 35, P 〈 0. 05 ）, invasion depth （t = 2. 50, P 〈 0. 05） and UICC stage （t = 3.40, P 〈 0. 05）. The median time of disease-free survival and overall survival were 28 months and 37 months. Kaplan-Meier survival analysis demonstrated that the disease free survival as well as the overall survival for the group with the higher density of T-bet positive lymphocyte was significantly better than the group with lower T-bet positive lymphocyte （χ^2 = 3.91, P 〈 0. 05 and χ^2 = 4. 00, P 〈 0. 05, respectively ）. COX multivariate analysis showed that, after regulating age, sex, tumor location, depth of invasion, tumor size and UICC stage, the high infiltration group of T-bet positive lymphocyte had the trend of recurrence [ FIR = 0. 56, 95% confidence interval （95% CI） = 0. 32- 0. 98, P 〈 0. 05 ] and death （ FIR = 0. 55, 95% CI = 0. 30-0. 99, P 〈 0. 05 ） risk reduction in gastric cancer patients, the difference was statistical significance. Conclusion The number of T-bet positive lymphocyte in gastric carcinoma was positively correlated with patient＇ s survival, which supports the notion that the T- bet was a positive regulator, which played an important role in tumor micro-environment.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第5期1051-1053,F0004,共4页
Chinese Journal of Experimental Surgery
基金
基金项目：：国家自然科学基金资助项目（81171653、30972703）
江苏省自然科学基金资助项目（BK2011246、BK2011247）
常州市科学技术局科技支撑计划资助项目（CE20125017）
常州市国际科技合作资助项目（CZ20110024）
常州市科技局生物医药科技专项资助项目（CJ20112020）
关键词
胃癌
T—bet
转录因子
免疫组织化学
Gastric carcinoma
T-bet
Transcription factor
Immunohistochemistry
