摘要
目的观察血清、转化生长因子（TGF）-β3、血小板衍生生长闪子（PDGF）-AB、骨形态发生蛋白（BMP）-2、成纤维细胞生长因子（FGF）对兔滑膜间充质干细胞（SMSCs）迁移的影响。方法选取含浓度0．1％、0．5％、1．0％、2．0％、5．0％、10．0％、20．0％血清以及含50μg/LTGF-β3、PDGF—AB、BMP-2、FGF的低糖DMEM作用于兔SMSCs，Transwell小室检测其迁移能力。结果 血清、TGF-β3、PDGF—AB、BMP-2、FGF均可趋化兔SMSCs迁移，血清浓度为5．0％时趋化作用较其他浓度组明显，每×100倍视野下迁移细胞数为（169．00±21．74）个，4种因子中PDGF—AB趋化作用较TGF—β3、BMP-2、FGF明显（219．83±11．29比84．50±10．33、146．67±17．91、99．37±12．27）。结论兔SMSCs可在TGF-β3、PDGF—AB、BMP-2、FGF作用下迁移，其中PDGF—AB趋化最明显，可用于促进SMSCs迁移归巢，进行软骨原位冉生的修复研究。
Objective To explore the chemotactic migration effects of serum, transforming growth factor （TGF）-β3, platelet derived growth factor （PDGF）-AB, bone morphogenetic protein （BMP）-2, and fibroblast growth factor （FGF） on rabbit synovium-derived mesenchymal stem cells （SMSCs）. Methods The SMSCs were obtained with passage isolation, and then stimulated by low-glucose DEME containing TGF-β3, PDGF-AB, BMP-2, FGF with 50 p^g/L concentration as well as 0. 1% , 0. 5% , 1.0% , 2.0% , 5.0%, 10. 0%, 20. 0% serum. The migration of SMSCs under five different ehemotaetic factors was observed in Transwell chamber assays. Results TGFq33, PDGF-AB, BMP-2, FGF and serum consistently enhanced the migration activity of rabbit SMSCs. Compared with other concentration, 5.0% is the appropriate concentration for serum to induce the migration of rabbit SMSCs. The average numbers of migratory cells （SMSCs） at × 100 magnification was 169. 00 ±21.74 in lower chamber under the 5.0% serum within the Transwell assay. Among the four growth factors, PDGF-AB showed more potent chemotactic effect than otherthree （219.83 ±11.29 vs. 84.50±10.33, 146.67±17.91, 99.37 ±12.27）. Conclusion The migration of SMSCs from rabbit could be enhanced by TGF-β, PDGF-AB, BMP-2 and FGF. PDGF-AB is the most potent chemotactic factor among the four growth factors, and may he useful to promote cell homing and cartilage regeneration in situ during cartilage repair.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第5期1013-1015,共3页
Chinese Journal of Experimental Surgery
基金
基金项目：国家自然科学基金资助项目（81071468、81171671）
关键词
滑膜间充质干细胞
细胞因子
趋化
迁移
Synovium-derived mesenchymal stem cells
Cytokine
Chemotaxis
Migration
