摘要
以葡萄糖为碳源,采用溶剂热法合成荧光碳点。用紫外-可见分光光度法和荧光光谱法研究了荧光碳点的发光特性。并将其与鼠抗人CD3抗体、羊抗鼠IgG抗体连接制备两种水溶性复合探针,对人血淋巴细胞进行免疫荧光标记成像。结果表明用该探针对人血淋巴细胞成像清晰,72h后细胞仍然保持明亮的荧光。
Fluorescent carbon dots （CDs） were synthesized by a solvothermal method with glucose as the carbon source and studied by UV-Vis absorption and fluorescence emission spectra. The as-prepared CDs were linked to mouse anti-human CD3 and immunoglobulin （IgG） for the labeling of fixed Humun T-lymphocyte. Experimental results have shown that CDs-based probes are excellent biomarkers for T-lymphocyte and the fluorescent microscopical images of human T-lymphocyte still exhibited bright fluorescence after 72 hours.
出处
《光谱实验室》
CAS
2013年第3期1035-1038,共4页
Chinese Journal of Spectroscopy Laboratory
基金
辽宁省自然基金资助项目(20102221)
辽宁省教育厅资助项目(L2010542)
辽宁省教育厅杰出青年学者成长计划(LJQ2011109)
沈阳市科技局资助项目(F11-262-9-21)
沈阳医学院优秀人才项目(No.20113068)
关键词
荧光碳点
免疫荧光标记
人血淋巴细胞
Fluorescent Carbon Dots
Immunolabeling
Human T-lymphocute
