摘要
目的:探讨切除修复交叉互补基因1(ERCC1)第118位密码子(C118T)和着色性干皮病基因D(XPD)第751位密码子(Lys751Gln)的单核苷酸多态性(SNPs),与晚期非小细胞肺癌(NSCLC)患者对含铂类药物化疗疗效的关系。方法:聚合酶链式反应-限制性片段长度多态性(PCR-RFLP)技术检测78例NSCLC患者的ERCC1(C118T)和XPD(Lys751Gln)基因型。结果:ERCC1(C118T)SNPs与铂类化疗疗效有关,P<0.05。C/C型化疗有效者7例(8.9%),C/T型为11例(14.1%),T/T型2例(2.6%);C/C化疗无效41例(52.6%),C/T型17例(21.8%),T/T型0;两组差异有统计学意义,P=0.003。T等位基因(T/T+C/T)型与C等位基因(C/C)型的化疗疗效差异亦有统计学意义,P=0.005,比值比(OR)=0.223,95%的可信区间(CI)为0.076～0.657;XPD(Lys751gln)SNPs与含铂类药物化疗疗效无关。ERCC1(C118T)、XPD(Lys751Gln)SNPs分布与年龄、性别、吸烟史、临床分期、体力状况评分(ECOG)和病理类型无关,P>0.05。结论:ERCC1(C118T)含有T等位基因,可作为预测晚期NSCLC患者铂类药物化疗敏感性的预测指标。
OBJECTIVE： To investigate whether SNPs of ERCC1 （118） and XPD （751） were associated with the sensitivity to platinum-based chemotherapy in advanced non-small cell lung cancer patients in Chinese population,and ex- plore its theoretical evidence to guide cisplatin based individualized chemotherapy for NSCLC patients. METHODS： SNPs of ERCC1 and XPD were detected by the polymerase chain reaction-restrictive fragment length polymorphism （PCR- RFLP） method. RESULTS： The polymorphic genotypes of ERCC1 （Cl18T） were related to chemotherapy sensitivity （P〈0.05）. Among responders,the allele frequencies of homozygous wild type（C/C） ,heterozygous （C/T） ,and homozy- gous variant （T/T） were 7（8.9%）,11（14.1%） and 2（2.6%）respectively. The corresponding genotypes in the nonre- sponders were 41（52.6%）,17（21.8%） and 0 respectively. The difference between the 2 groups was statistically signifi- cant （P=0. 003）. After combining the T/T and C/T genotypes, the difference remained statistically significant （P = 0.005）. The odds ratio （OR） for responders was 0. 223 （P=0. 005） and 95% confidence interval （CI） was between 0. 076 and 0. 657, but XPD （Lys751gln） were significantly related to chemotherapy sensitivity. SNPs of ERCC1 and XPD are not associated with age, sex, histologic type, ECOG performance status, smoking history and clinical tumor stage （P〈 0.05）. CONCLUSION：patients carrying at least 1 T allele （T/T C/T） were more likely to be the responders to chemo- therapy compared with those who carrying the C allele.
出处
《中华肿瘤防治杂志》
CAS
北大核心
2013年第10期726-729,共4页
Chinese Journal of Cancer Prevention and Treatment
基金
国家自然科学基金(81272610)
苏州市社会发展指导计划(SZD0877)
苏州市科技局(YJS0925)
关键词
切除修复交叉互补基因1
着色性干皮病基因D
顺铂
基因多态性
非小细胞肺癌
excision repair cross-complementing complementation group l^xeroderma pigmentosum group D
cispla-tin
genes polymorphism
cancer, non-small cell lung
