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猪繁殖与呼吸综合征病毒延边地方株GP4结构基因分析 被引量:3

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摘要 猪繁殖与呼吸综合征病毒(PRRSV)易引起基因变异现象,使得异地疫苗不能有效控制本地区猪繁殖与呼吸综合征(PRRS)的发生。为研制适合本地区的亚单位疫苗,参照GenBank中已发表的PRRSV序列设计了1对特异性引物,对吉林省延边地区分离株YB-0605 GP4基因进行了序列扩增和测序,序列分析结果表明,PRRSV YB-0605株GP4氨基酸序列与CH-1α、HEB1株氨基酸序列高度一致,而与VR-2332、BJ-4株有一定差异,和LV株在N端及中心区具有很大差异,YB-0605株GP4蛋白抗原性及亲水性与河北省株具有较高相似性;与VR-2332株相比,GP4蛋白在第30~40位氨基酸处抗原表位形态发生较大变化,在第50位氨基酸处抗原表位优势增强现象。
出处 《江苏农业科学》 北大核心 2013年第4期14-17,共4页 Jiangsu Agricultural Sciences
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同被引文献39

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