摘要
目的建立用自制的鼠尾胶原联合胰酶消化法原代培养SD新生大鼠耳蜗螺旋神经元。方法采用出生3 d内的SD大鼠,取螺旋神经节组织,通过胰酶消化后在自制鼠尾胶原包被的培养皿中进行原代培养,应用神经微丝蛋白进行免疫组化鉴定。结果获得的螺旋神经元细胞在体外能较快地贴壁,可以存活、生长,细胞形态良好。结论自制鼠尾胶原联合胰酶消化的培养方法可获得良好状态的螺旋神经元细胞,为原代培养螺旋神经元提供了新思路。
Objective To establish a culture model of spiral ganglion neurons （SGN） of newborn rats in vitro with application of self-made rat-tail collagen combined trypsin digestion method. Methods The culture of SGN from newborn rats within 3 d was carried out.The SGN tissues were taken and performd the primary culture in the culture dish coating with self-made rat-tail collagen. The immunohistochemical identification of cultured SGNs was performed by neurofilament protein（NFP）. Results On the condition in vitro, acquired SGNs had rapid adherence, could survive and grew with good cellular morphology. Conclusion SGN can be cultured in vitro by using trypsin digestion combined self-made rat-tail collagen,which provides a new thinking for SGN primary culture.
出处
《现代医药卫生》
2013年第8期1170-1171,共2页
Journal of Modern Medicine & Health
