摘要
目的本研究采用高脂饮食诱导肥胖大鼠模型，观察肾小球足细胞Podocin的变化及其在导致蛋白尿中的作用。方法实验大鼠分正常对照组（Con组）和肥胖组（Ob组）。在造模14周时，分别检测体重、身长、尾长、肾重、尿蛋白、尿RBP、尿肌酐、血糖、血脂、血尿酸（uA）、血肌酐（Cre）、血尿素氮（BUN）及血浆胰岛素，计算Lee’s指数、组织胰岛素抵抗、胰岛素敏感性指数、内生肌酐清除率（Ccr）、相对肾重，用光学显微镜、电子显微镜等观察肾脏形态变化，用免疫组织化学和Westernblot方法，检测肥胖大鼠肾小球足细胞Podocin的表达。结果0）0周时0b组与Con组比较性别、体重、身长、尾长，Lee’S指数等差异无统计学意义（P〉0．05）；14周时Ob组体重、身长，Lee’s指数明显高于Con组，差异有统计学意义（P〈0．05）。②14周时0b组与Con组比较空腹血糖升高，高密度脂蛋白降低，差异有统计学意义（P〈0．05）。2组间uA、Cre、BUN等差异无统计学意义（P〉0．05）。③0b组在14周时尿蛋白和Ccr明显高于Con组，差异有统计学意义（分别P〈0．05，P〈0．01）。④Ob组与Con组比较肾重无明显差异，Ob组相对肾重〈Con组（P〈0．05）。⑤光镜下0b组可见肾皮质区肾小球肥大，肾小囊狭窄，节段性毛细血管闭塞，球囊粘连。电镜下Ob组肾脏肾小球足细胞呈粗大状，足突轻度微绒毛化。⑥免疫组化染色和Westernblot检测0b组肾小球足细胞Podocin蛋白表达比Con组减少，差异有统计学意义（t值为17．24；P〈0．001）。结论肥胖大鼠肾小球足细胞Podocin蛋白的表达减少，伴有足细胞超微结构的改变，可能是其产生蛋白尿的原因之一。
Objective To discuss the mechanism of obesity related glomerularpathology, we detect the expression of Podocin in glomerular podocytes of obesity rats induced by high fat diet. Methods The rats were divided into 2 groups ： normal control group（ Con group） and obesity group（ Ob group）. At the 14th week of mold- ing, the body weight, body long, tail long, kidney weight, uria microalbumin, RBP, uria Cre, blood glucose, cholesterol, triglyceride, HDL-C, UA, Cre, BUN, and insulin were measured. The Lee＇s index, insulin resist- ance index, insulin sensitive index, Ccr and relative kidney weight were calculated. The tissue structures of the kidney of both groups were studied under light microscope and transmission electro microscope. The expression of Podocin in podocyte of rats＇ kidney was measured by immunohistochemistry and western blot. Results （1）The difference had no statistical significance between the 2 groups in gender, weight, body length, tail length and Lee＇s index at the beginning（P 〉 0. 05 ）. But after 14 weeks, the weight, body length and Lee＇s index of Ob group were significantly higher than those of Con group（P 〈0. 05）. （2）At the 14th week, the fasting plasma glu- cose of Ob group was higher than that of Con group （ P 〈 0. 05 ）, and the high density lipoproteins （HDL） of Ob group was lower than that of Con group（P 〈 0. 05 ）. However, the difference had no statistical significance in u- fine acid（ UA）, serum Cre and blood urea nitrogen（BUN） between the 2 groups（ P 〉 0.05 ）. （2）The urine protein and endogenous creatinine clearance rate （Ccr）in Ob group were higher than those of Con group （P 〈 0.05, P 〈 0. 01）. （4）The relative kidney weight of Ob group was lower than that of Con group（P 〈0. 05）. （5）For rats in Ob group, glomerular hypertrophy, the Bowman＇ capsule stricture, abnormality in the segmental capillary wall and extraglomerular mesangial cell were observed under light microscope. The glomerular podo
出处
《中华内分泌外科杂志》
CAS
2013年第2期144-149,共6页
Chinese Journal of Endocrine Surgery
基金
吉林省科技发展计划项目（200905119）
