摘要
以分离筛选后的昭通乌天麻野生蜜环菌为试材,以PDA为基本培养基,研究了不同浓度NAA对昭通乌天麻野生蜜环菌菌丝出现时间、菌索出现时间、菌索长度、生物量以及生长状况的影响。结果表明:处理4(5.0μg/mL)的菌丝、菌索出现最快;处理1～5(0.5、1.0、2.5、5.0、10.0μg/mL)菌索长度明显大于对照,处理6～9(25、50、100、250μg/mL)菌索生长明显小于对照;最终的生物量以处理4(5.0μg/mL)为最大;处理3(2.5μg/mL)和处理4(5.0μg/mL)蜜环菌生长状况最好。综合比较来看,以处理4(5.0μg/mL)为蜜环菌生长的最佳浓度。该研究为后续蜜环菌的培养、菌包生产以及研发奠定了一定的基础。
Taking Armillaria rnellea of Gastrodia elata Blume isolated and screened as the experimental material, the effects of different concentrations of NAA on the mycelium and rhizomorph emergence date, rhizomorph length, biomass and growth status of Armillaria mellea o f Gastrodia elata Blume were investigated by using ten different concentrations of NAA added in the PDA basal culture media. The results showed that the mycelium and rhizomorph emergence dates of No. 4（5. 0μg/mL） treatment were earlier than those of other treatments ; the rhizomorph length of treatments from 1 to 5（0. 5,1.0,2. 5,5.0,10. 0 μg/mL） were obviously longer than CK,while treatments from 6 to 9（25.50,100,250μg/mL） were much worse than CK; the biomass of No. 4 treatment was the highest among all the treatments; and the growth status of No. 3 and 4 treatments was much better than others. On the basis of comprehensive comparison and analysis, No. 4 treatment was the best one for the growth of Arrnillaria mellea, which laid some foundation for the cultivation, production,research and exploitation of Armillaria mellea in the future.
出处
《北方园艺》
CAS
北大核心
2013年第9期169-171,共3页
Northern Horticulture
基金
云南省自然科学基金资助项目
云南省园林植物与观赏园艺省级重点学科
云南省高校园林植物与观赏园艺重点实验资助项目
关键词
蜜环菌
菌丝
菌索
NAA
生长状况
Armillaria rnellea
mycelium
rhizomorph
NAA
growth status
