摘要
目的:研究河北产蜂胶不同溶剂提取液对金黄色葡萄球菌、表皮葡萄球菌的体外抑菌作用。方法:采用微量板法对金黄色葡萄球菌、表皮葡萄球菌进行培养,分别加入不同溶剂(95%乙醇、75%乙醇、纯化水)提取的蜂胶溶液,观察抑菌效果,测定最低抑菌浓度(MIC)。结果:蜂胶95%乙醇提取液对金黄色葡萄球菌与表皮葡萄球菌的MIC均为256μg/ml;蜂胶75%乙醇提取液对金黄色葡萄球菌与表皮葡萄球菌的MIC均为128μg/ml;蜂胶水提取液对金黄色葡萄球菌与表皮葡萄球菌均无抑菌作用。结论:河北产蜂胶醇提取液对痤疮主要致病菌有较好的体外抑菌效果,蜂胶75%乙醇提取液的抑菌效果优于蜂胶95%乙醇提取液。
OBJECTIVE ： To study the antibacterial activities of propolis extract from Hebei province on the Staphylococcus aureus and Staphylococcus epidermidis in vitro. METHODS： Staphylococcus aureus and Staphylococcus epidermidis were cultured by microplating method, and propolis solution was extracted with different solvents （95% ethanol, 75% ethanol, purified water）. The bacteriostatic action of propolis extract was observed, and MIC was determined. RESULTS： The MIC of propolis extracted by 95% ethanol against Staphylococcus aureus and Staphylococcus epidermidis were 256 μg/ml; the MIC of propolis extracted by 75% alcohol against Staphylococcus aureus and Staphylococcus epidermidis were 128 μg/ml; water extracts had no bacteriostatic action against Staphylococcus aureus and Staphylococcus epidermidis. CONCLUSIONS： Ethanol extracts of propolis from Hebei province show satisfactory bacteriostatic action against the major pathogenic bacteria of acne, and propolis extracted by 75 % ethanol have better bacteriostatic action than propolis extracted by 95 % ethanol.
出处
《中国药房》
CAS
CSCD
2013年第19期1742-1743,共2页
China Pharmacy
基金
河北省中医药管理局科研计划项目(No.2009034)
