摘要
将模式生物毒性预筛与传统啮齿类动物实验相结合是今后国际化学品毒性筛检和安全性评估领域的发展方向。应用模式生物秀丽隐杆线虫(Caenorhabditis elegans)进行化学品急性毒性评估的指标分为致死性指标和亚致死性指标,其中,致死性指标包括半数致死浓度和半数致死时间,亚致死性指标包括生长、发育、繁殖及运动、行为等指标。基于转基因线虫的生物标志物指标提高了检测灵敏度,近年来发展了使用微流控芯片及复杂目标参数分析和分选系统等的高通量、快速化学品毒性评估技术。该文对上述研究进展作一综述。
It is a new development direction that toxicity pre-screening using model organisms combined with traditional rodent animal test in the field of chemical toxicity screening and safety assessment. The chemical acute toxicity assay using mode organism Caenorhabditis elegans includes both lethality and sublethality endpoint. Lethality methods use median lethal concentration （LC50） and median lethal time （LT50） as endpoint. Sublethality endpoint involves the use of growth, development, reproduction, movement and behavior. Transgenic strains with specific biomarkers can improve the detection sensitivity. High throughput toxicity assay methods have been developed in recent years ,such as microfluidic chips and Complex Object Parametric Analyzer and Sorter （COPAS）. In this paper, recent developments of acute toxicity testing and evaluation of chemicals based on Caenorhabditis elegans were reviewed.
出处
《环境与健康杂志》
CAS
CSCD
北大核心
2013年第4期366-370,共5页
Journal of Environment and Health
基金
国家自然科学基金(81273108)
首都卫生发展科研专项(首发2011-1013-03)
北京市卫生系统高层次卫生技术人才培养项目(2011)
北京市优秀人才培养项目(2013)
关键词
秀丽隐杆线虫
急性毒性
化学品
Caenorhabditis elegans
Acute toxicity
Chemical
