摘要
目的评价鞘内注射利多卡因对大鼠丙泊酚镇静效应和脑组织利多卡因含量的影响。方法雄性sD大鼠40只，体重250～350g，2～3月龄，2采用随机数字表法，将其分为4组（n=10）：正常对照组（C组）、静脉注射利多卡因组（IV-L组）、鞘内注射生理盐水组（IT-NS组）和鞘内注射利多卡因组（TI-L组）。IT-Ns组和IT-L组分别鞘内注射生理盐水15μl和2％利多卡因15μl，IV-L组静脉注射2％利多卡因15μl。在鞘内给药或静脉给药后10min静脉输注丙泊酚，记录大鼠眼睑反射消失时丙泊酚剂量，IT-L组和IV-L组取脑组织，采用反相高效液相色谱法测定利多卡因水平。结果与c组、IV-L组和IT-Ns组比较，IT-L组眼睑反射消失时丙泊酚剂量降低（P〈0．05），c组、IV-L组和IT-Ns组间眼睑反射消失时丙泊酚剂量比较差异无统计学意义，IV-L组与IT-L组间脑组织利多卡因含量比较差异无统计学意义（P〉0．05）。结论大鼠鞘内注射利多卡因产生镇静效应的机制与其对与脑的直接作用无关。
Objective To investigate the effects of intrathecal （IT） lidocaine on propofbl-induced sedation and content of lidocaine in brain tissues in rats. Methods Twenty male Sprague-Dawley rats, aged 2-3 months, weighing 250-350 g, were equally and randomly assigned to one of four groups： control group （group C）, iv lido- eaine group （group IV-L）, IT normal saline group （group IT-NS） and IT lidocaine group （group IT-L）. Groups IT-NS and IT-L received IT normal saline 15 μl and 2% lidocaine 15 μl, respectively. Group IV-L received iv 2% lidocaine 15 μl. Propofol was infused starting from 10 min after IT or iv administration. When the eyelash reflex disappeared, the infusion of propofol was stopped and the dose of propofol consumed was recorded. The rats were sacrificed in IT-L and IV-L groups and brains were removed for determination of lidoeaine level in brain tissues （by RP-HPLC） .Results Compared with groups C, IV-L and IT-NS, the dose of propofol consumed when the eyelash reflex disappeared was significantly decreased in gro.up IT-L （P 〈 0.05）. No significant difference was found in the propofol requirement when the eyelash reflex disappeared between groups C, IV-L and IT-NS and in the content of lidoeaine in brain tissues between groups IV-L and IT-L （ P 〉 0.05） . Conclusion Sedation induced by lido- eaine administered intratheeally is not due to a direct action of lidoeaine on the brain in rats.
出处
《中华麻醉学杂志》
CAS
CSCD
北大核心
2013年第2期220-222,共3页
Chinese Journal of Anesthesiology
基金
宁夏自然科学基金（NZ08114）
宁复医学院特殊人才启动项目（XT200718）
关键词
利多卡因
注射
脊髓
二异丙酚
清醒镇静
脑
Lidocaine
Injections, spinal
Propofol
Conscious sedation
Brain
