摘要
目的应用ELISA法分析结核分枝杆菌抗原模拟肽对结核病的诊断价值。方法根据噬菌体筛选得到的结核分枝杆菌抗原模拟肽序列进行人工合成,间接ELISA法检测合成短肽诊断结核病的灵敏度和特异性。结果人工合成的2条12肽(T1和T2),经间接ELISA初步鉴定,显示其敏感性和特异性分别为67.9%、50.0%和100.0%、96.4%。结论合成短肽T1对结核病有着较好的诊断价值。
Objective To evaluate the value of the mimic peptides of Mycobacterium tuberculosis in diagnosing tuberculosis by ELISA. Methods The highly frequent mimic peptides of Mycobacterium tuberculosis were synthesized based on their amino acid sequences. The sensitivity and specificity of the synthetic short peptides in diagnosing tuberculosis were detected by indirect ELISA. Results The sensitivities and specificities of the synthetic short peptides （T1 and T2） were 67.9 %, 50.0 % and 100.0 %, 96.4 % respectively. Conclusions The antigen mimic peptide T1 has great diagnostic value for tuberculosis.
出处
《实用预防医学》
CAS
2013年第4期411-414,共4页
Practical Preventive Medicine
基金
湖南省高等学校科学研究项目(10C0920)
湖南省研究生科研创新项目(CX2010B231)
关键词
结核分枝杆菌
模拟肽
间接ELISA
诊断
Mycobacterium tuberculosis
Mimic peptide
Indirect ELISA
Diagnosis
