摘要
目的了解在体外培养条件下人源性免疫球蛋白G(IgG)刺激人胶质瘤细胞系U87MG后,对增殖细胞核抗原(PCNA)、激活的细胞外信号调节激酶(P-ERK)及激活的非受体酪氨酸激酶(P-SRC)表达的影响。方法用不同浓度的IgG(0.01、0.1、1μg/mL)刺激U87MG,24h后采用Western blot方法检测PCNA、ERK及P-ERK、SRC及P-SRC的表达变化情况。结果 IgG直接作用于体外培养的胶质瘤U87MG后,相对于对照组而言PCNA、P-ERK及P-SRC表达有明显的升高,并且表达呈现出一定的剂量依赖性。结论 IgG可能与神经系统癌症有关联。
Objective To analyze the effect of immunoglobulin G （IgG） on PCNA, P-SRC and P-ERK expres- sion in Glioma cell lines U87MG. Methods By Western blot, we detected PCNA, P-SRC and P-ERK expression in Glioma cell lines U87MG at 24h after different concentration of IgG stimulation. Results The result of western blot showed that after IgG stimulation for 24 hours, PCNA, P-SRC and P-ERK expression increased in U87MG. Mean- while, the result also showed that PCNA, P-SRC and P-ERK expression increased dose-dependently in the IgG groups. Conclusion IgG can increase PCNA, P-SRC and P-ERK expression in Glioma cell lines U87MG, which in dicated IgG probably was relative to nerve system cancer.
出处
《检验医学与临床》
CAS
2013年第8期936-937,939,共3页
Laboratory Medicine and Clinic
