摘要
目的:比较不同干燥方法对灰毡毛忍冬花蕾中4种活性成分含量的影响。方法:采用生晒法、烘干法、蒸制生晒法和蒸制烘干法对灰毡毛忍冬花蕾进行干燥处理。参照《中国药典》,利用高效液相色谱(HPLC)-紫外扫描(UV)法测定干燥样品中绿原酸和木犀草苷的含量;利用HPLC–蒸发光散射检测器(ELSD)法测定干燥样品中灰毡毛忍冬皂苷乙和川续断皂苷乙的含量。结果:蒸制烘干法处理的灰毡毛忍冬花蕾中绿原酸含量和皂苷总量最高,分别为3.705%和11.834%,木犀草苷含量为0.103%,均高于《中国药典》对山银花和金银花指标物质含量的规定。结论:不同干燥方法对灰毡毛忍冬花蕾中活性成分的含量有较大影响,以蒸制烘干法最优。
Objective： To compare the effect of different dehydrating methods on the contents of four active constituents in flower buds of Lonicera macranthoides. Methods ： Dehydrating methods of sun drying, oven drying, steamingsun drying and steaming-oven drying were chosed to deal with flower buds of Lonicera macranthoides. According to Chinese Pharmacopoeia, the contents of chlorogenic acid and ga- luteolin in dried samples were determined by High Performance Liquid Chromatography （HPLC） -UV, and the contents of macranthoidin B and dipsacoside B were determined by HPLC - Evaporative Light Scattering Detector （ELSD）. Results ： Using the steaming - oven drying method, the contents of chlorogenie acid, total saponins and galuteolin from dried flower buds reached 3. 705% , 11. 834% and 0. 103% , respectively, which were higher than the prescribed contents of corresponding index in Chinese Pharmacopoeia. Conclusion： Different dehydrating methods had great influence on the contents of active constituents in flower buds of Lonicera macranthoides. The steaming - oven drying method performed optimally.
出处
《中国野生植物资源》
2013年第2期8-11,共4页
Chinese Wild Plant Resources
基金
"十二五"国家科技支撑计划(2011BAD33B02)
常州市科技支撑计划(CE20115054)
