摘要
目的观察5-氟尿嘧啶（5-Fu）和顺铂（cP）联合使用对肿瘤细胞是否具有协同抗增殖的作用。方法利用蛋白组织学分析，包括双向凝胶电泳及基质辅助激光解吸／离子化-飞行时间质谱来探索5-Fu或／和CP治疗后与抗增殖相关的蛋白。结果5-Fu处理后有25种蛋白上调节和16种蛋白下调，CP处理后有20种蛋白上调和11种蛋白下调。5-Fu和CP联合使用的最佳浓度为5-Fu：0．5μmol/L和CP：1．0μmol／L，两者联合使用72h后出现协同效应，其协同效应是通过调节膜凋亡受体通路和线粒体凋亡通路而实现的，同时联合使用2种药物后宫颈癌细胞凋亡的敏感性提高。结论5-Fu和CP联合使用可通过协同诱导细胞凋亡而抑制宫颈癌细胞的增殖。
Objective - To investigaie the synergistic effect of 5-fluorouracil （5-Fu） and cisplatin on cervical carcinoma cells in vitro. Methods Using proteomics analyses, including two-dimensional gel electrophoresis and matrix assisted laser desorption/ionization-time of flight mass spectrometry （ MALDI- TOF-MS）, we investigated the antiproliferative effect-related proteins after treatment with 5-Fu and/or CP. Results It was found 25 proteins were up-regulated and 16 down-regulated after 5-Fu treatment as deter- mined by spot volume, and 20 proteins were up-regulated and 11 down-regulated after CP treatment as deter- mined by spot volume. The optimal concentrations for combined use of 5-Fu and CP were as follows： 5-Fu： 0. 5 μmol/L, and CP： 1.0 pLmol/L, and the synergistic effects occurred after 72 h. The combination of 5-Fu and CP engaged two apoptotic pathways including the membrane death receptor-mediated apoptosis pathway and the mitochondrial apoptotic pathway. Moreover, the combination of 5-Fu and CP resulted in a remarkably increasing susceptibility to apoptosis. Conclusion The combination of 5-Fu and CP suppresses the growth of cervical carcinoma cells by synergistic effect with the induction of apoptosis. In vitro the synergistic effect of 5-Fu and CP may provide the theoretical guidance for clinical treatment of cervical cancer patients.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2013年第4期818-820,共3页
Chinese Journal of Experimental Surgery
关键词
宫颈癌
5-氟尿嘧啶
顺铂
蛋白组学
Cervical carcinoma
5-fluorouracil
Cisplatin
Proteomics analysis
