摘要
目的观察磁化胆道支架在磁靶向治疗胆管癌中的作用。方法建立皮下异位胆管癌移植瘤裸鼠模型32只,随机平分成4组,A组:为实验组,采用自制的胆道磁性支架丝,在肿瘤内部建立300高斯(Gs)的磁场,尾静脉注射5-Fu纳米磁小体;B:空白对照组,肿瘤模型自然生长,无磁场和药物应用;C:单纯磁化胆道支架组,建立与A组一致的肿瘤局部内磁场,无药物治疗;D:外磁场组,建立5000 Gs的肿瘤局部外磁场,药物干预同A组。测各组抑瘤率,并观察肿瘤组织病理变化。结果与空白对照组比较,A、C、D组的肿瘤抑制率分别为40.120%、18.039%、26.078%,实验组肿瘤生长受到明显抑制,与B、C、D组比较差异有统计学意义(P<0.05),该组肿瘤组织镜下显示大量细胞凋亡,可见大量纳米磁小体颗粒沉积在凋亡的肿瘤细胞内。结论磁化胆道支架联合磁性纳米药物可靶向抑制肿瘤生长,其基于内磁场的磁靶向治疗效果优于传统的依靠外磁场的靶向治疗方法。
Objective To detect the effect of target to cholangiocarcinoma treated by magnetic biliary stent combining with magnetic nanoparticle containing 5-fluorouracil （5-Fu） ;Methods 32 modes bearing tumor were divided equally into four groups on random including： （a） group A consisted of internal magnetic field built by magnetic bihary stent wires inserting into tumor tissue and received magnetic nanoparticles containing 5-Fu administered via tail vein injection at 250mg/kg on consecutive five days; （b） group B received placebo （ sodium chloride） ; （c） group C received pure magnetic biliary stent without the applying of magnetic nanoparticles; （d） group D consisted of external magnetic fields and the same treatment of magnetic nanoparticles containing 5-Fu as group A. determin tumor inhibition rate Tumor tissues were observed by transmission electron microscope. Results Contrast with control group ,The tumor inhibition rate of group A, C and D was 40. 120%, 18. 039% ,26. 078% respectively, The difference between experimental group （A group） and group C ,D showed significant. More apoptosis of tumor cells could be detected easily in group A. Conclusion Magnetic hiliary stem combining with magnetic nanoparticle containing 5-fluorouracil could inhibit the growth of cholangiocarcinoma, and its curative effect is better than the traditional method based on external magnetic fields.
出处
《中华实验外科杂志》
CAS
CSCD
北大核心
2007年第8期936-938,共3页
Chinese Journal of Experimental Surgery
基金
国家高技术研究发展计划(863计划)资助项目(2002AA214061)
关键词
纳米粒
化疗
胆道
支架
胆管癌
Nanoparticle
Chemotherapy
Biliary
Stem
Cholangiocarcinoma
