摘要
目的评价内磁场在磁靶向治疗胆道恶性肿瘤中的作用。方法建立异位胆管癌移植瘤裸鼠模型32只,随机分成4组,每组8只。A组(实验组):采用自制的胆道磁性支架丝,在肿瘤内部建立30mT的磁场,尾静脉注射纳米氟尿嘧啶(5-FU)磁小体;B组(空白对照组):肿瘤模型自然生长,无磁场和药物应用;C组(单纯内磁场组):建立与A组一致的肿瘤局部内磁场,无药物治疗;D组(外磁场组):建立500mT的肿瘤局部外磁场,药物干预同A组。在首次治疗当天和治疗后第4、7、11、15、19天,分别测各组肿瘤体积,做重复测量的多因素方差分析。观察期结束后处死裸鼠,观察肿瘤组织病理变化。结果与空白对照组比较,单纯内磁场组、外磁场组和实验组的肿瘤抑制率依次为18.039%、26.078%、40.12%,实验组肿瘤生长受到明显抑制,与3个对照组的肿瘤体积均有显著性差异,该组肿瘤组织镜下显示大量细胞凋亡,可见大量纳米磁小体颗粒沉积在凋亡的肿瘤细胞内。结论基于内磁场的磁靶向治疗可抑制肿瘤生长,其效果优于传统的依靠外磁场的靶向治疗方法。
Objective To evaluate tile effect of magnetic nanoparticle containing 5-fluorouracil （5-FU） targeting in treating cholangiocarcinoma based on internal magnetic field built inside the tumor. Methods Thirty-two modes cancer bearing expeimental animals were divided equally into four groups randomly; group A consisted of internal magnetic field built inside tumor tissue and were treated with magnetic nanoparticles containing 5-FU administered via tail vein injection of 250mg/kg on five consecutive days; group B received placebo （sodium chloride）; group C received pure internal magnetic fields without the applying of magnetic nanoparticles; group D consisted of external magnetic fields and the same treatment of magnetic nanoparticles containing 5-FU as in group A. The tumor volume was measured every 3 days, six times in total from the day of the first treatment. Tumor tissues were observed by transmission electron microscope after the completion of observation periods. Results The experimental group （group A） showed significant curative effect than other three groups analyzed by Repeated Measures Analysis of Variance. Moreover, more apoptosis of tumor cells could be detected easily in group A. Conclusions Magnetic drug targeting based on internal magnetic field can inhibit the growth of tumor tissue, and its curative effect is better than the traditional method based on external magnetic fields.
出处
《北京医学》
CAS
2006年第12期726-729,共4页
Beijing Medical Journal
基金
国家863计划基金资助项目(编号2002AA214061)
关键词
磁靶向
磁性纳米颗粒
化疗
内磁场
胆管癌
Magnetic targeting Magnetic nanoparticle Chemotherapy Internal magnetic field Cholangiocarcinoma
