摘要
目的对螳螂共生真菌TG-3的次生代谢产物进行研究。方法采用硅胶柱色谱、Sephadex LH-20凝胶柱色谱、高效液相等方法对TG-3发酵液的乙酸乙酯提取物进行分离和纯化,并运用波谱学方法鉴定化合物的结构。结果从发酵产物中分离得到8个化合物,分别鉴定为1,4-dimethyl-3[(4'-γγ-dimethylallyloxyphenyl)methyl]-3,6-bis(meth-ylthio)piperasine-2,5-dione(1)、fusaperazine E(2)、cyclo-(Gly-Tyr)4,4-dimethylallyl ether(3)、bisdethiobis(methylthio)gliotoxin(4)、胶酶毒素(5)、didehydrobisdethiobis(methylthio)gliotoxin(6)、邻苯二甲酸二丁酯(7)、尿嘧啶(8)。结论以上化合物均为首次从TG-3中分离得到。
AIM To study the secondary metabolites from symbiotic fungus TG-3 associated with Tenodera aridifolia.METHODS The compounds were isolated from ethyl acetate extracts of symbiotic fungus TG-3 associated with Tenodera aridifolia fermentation liquid by different separation techniques,such as silica gel,Sphadex LH-20 column chromatography,and HPLC.The structures were elucidated by means of comprehensive spectroscopic methods.RESULTS Eight compounds were isolated and identified as 1,4-dimethyl-3[（4′-γγ-dimethylallyloxyphenyl）methyl]-3,6-bis（methylthio）piperasine-2,5-dione（1）,fusaperazine E（2）,cyclo-（Gly-Tyr）4,4-dimethylallyl ether（3）,bisdethiobis（methylthio）gliotoxin（4）,gliotoxin（5）,didehydrobisdethiobis（methylthio）gliotoxin（6）,phthalic acid dibutylester（7）,and uracil（8）.CONCULSION All of these compounds are obtained from symbiotic fungus TG-3 associated with Tenodera aridifolia for the first time.
出处
《中成药》
CAS
CSCD
北大核心
2013年第4期742-744,共3页
Chinese Traditional Patent Medicine
基金
中国博士后科学基金面上项目(2011M500898)
关键词
螳螂共生真菌
次生代谢产物
胶酶毒素
symbiotic fungus associated with Tenodera aridifolia
secondary metabolites
gliotoxin
