摘要
通过测定金银花枝叶提取物对猪繁殖与呼吸综合征病毒(PRRSV)感染的Marc-145细胞的最小保护浓度和对病毒感染滴度的影响来评价其体外抗猪繁殖与呼吸综合征病毒(PRRSV)的活性.结果显示,金银花枝叶95%乙醇提取物经D101型大孔树脂吸附后,得到的60%乙醇/水洗脱部位对PRRSV感染细胞的最小保护浓度为6.25μg/mL,6.25μg/mL 60%乙醇/水洗脱部位使PRRSV病毒滴度从105.8 TCID50降低至100.3 TCID50左右,即60%乙醇/水洗脱部位具有良好的体外抗PRRSV活性.利用溶剂提取法、正相硅胶柱层析、Sephadex LH-20和RP-HPLC等色谱法,从该有效活性部位中分离得到了5个化合物,通过理化性质和1 H NMR、13 C NMR等光谱数据鉴定化合物分别为Dihydrodehydrodiconiferyl alcohol 4′-O-β-D-glucoside(1),Apigenin 5-O-β-D-glu-copyranoside(2),Secoxyloganin(3),Benzyl alcohol O-(6′-O-β-D-xylopyranosyl)-β-D-glucopyr-anoside(4)和Sweroside(5).
The antiviral effect of the extracts of the Lonicera japonica leaves on PRRSV in vitro was evaluated by determining the minimum protection concentration on PRRSV infected Marc-145 cell and TCIDs0. The results showed that the 60% ethanol/water elution fraction possess the perfect protective effect on PRRSV infected cell and with the minimum protection concentration of 6.25 μg/mL. The 6.25μg/mL 60% ethanol/water elution fractions decreased PRRSV titer from 105.8 TCIDs0 to 100.3 TCID50. That is to say, 60% ethanol/water elution fractions of Lonicera japonica is a potential antiviral drug owing to its perfect anti-PRRSV effect. To study the chemical constituents of the Lonicera japonica, the air-dried branches and leaves of this plant were extracted with 95% ethanol. The extracts were separated and purified by normal-phase, Sephadex LH- 20 and RP-HPLC. Five compounds were obtained from the effective fractions anti- PRRSV of the branches and leaves of the Lonicera japonica and their structures were identified as Dihydrodehydrodiconiferyl alcohol 4r-O-β-D-glucoside （1）, Apigenin 5-O-β- D-glucopyranoside （2）, Secoxyloganin （3）, Benzyl alcohol O-（6＇-O-β-D-xylopyranosyl）- β-D-glucopyranoside （4） and Sweroside （5）.
出处
《华中师范大学学报（自然科学版）》
CAS
北大核心
2013年第2期213-217,共5页
Journal of Central China Normal University：Natural Sciences
基金
国家自然科学基金项目(31070313
21272136)
中华人民共和国水利部公益项目(SWCC2012003)
