摘要
以来自我国10个省(市)、自治区的540份甘蔗细茎野生种无性系为材料,根据采集地信息及其在20对SSR引物上的分子标记数据和15个表型性状资料,开展核心种质构建研究。不同取样量(5%、10%、20%、30%、40%、50%、60%、70%、80%和90%)分析表明,10%的取样比例可获得70%以上的变异保留比例,是较好的核心种质取样规模;对5种采集地分组取样策略(等量法、简单比例法、平方根比例法、对数比例法和多样性比例法)和2种无分组取样策略(最大变异保留法和随机抽样法)比较表明,简单比例法获得的核心种质代表性最好,为最优取样策略。最后,在简单比例法取样筛选出的54份核心样品中,又通过定向选择补充了6份具有优异表型性状的材料,构建了含60份无性系的甘蔗细茎野生种核心种质,分子和表型检验都表明本研究所构建的核心种质具有较好的代表性和遗传多样性。
Five hundred and forty accessions of Saccharum spontaneum L. from ten provinces of China were characterized using geographical information, molecular markers on 20 SSRs, and 15 agro-morphological traits to establish a core collection. Ten different sampling ratios of accessions were tested, including 5% and 10% to 90% with an interval of 10%. The results showed that 10% of samples could conserve more than 70% of variations, which indicated that 10% was an adequate sampling ratio for establishing the core collection. A comparison of the efficiencies of five sampling strategies by geographical grouping （constant, proportional, square root, logarithmic, and genetic diversity-depend） and two non-grouping sampling strategies （maximization and random sampling）, indicated proportional strategy was optimal in term of establishing the most representative core collection. Thus, a core collection of 60 accessions, including 54 selected based on proportional strategy and six accessions selected by their specific agro-morphological traits, was constructed. The tests on SSR data and agro-morphological traits demonstrated that the core collection have high genetic diversity and a good representative to the entire collection.
出处
《作物学报》
CAS
CSCD
北大核心
2013年第4期649-656,共8页
Acta Agronomica Sinica
基金
国家自然科学基金项目(30800700)
国家现代农业产业技术体系建设专项(CARS-20-1-4)
广东省科技计划项目(2011B060400019)资助
关键词
甘蔗细茎野生种
核心种质
SSR
表型性状
Saccharum spontaneum L.
Core collection
S SR
Agro-morphological traits
