摘要
采用RT -PCR技术 ,以根据国外报道的几种双子叶植物的甘油 - 3-磷酸转酰酶的相对保守的氨基酸序列而设计的简并引物作为扩增引物 ,从不同抗冷性水稻品种中均扩增到一段约 315bp的cDNA片段。测序结果表明它们都是编码GPAT的部分cDNA ,含有 315个核苷酸 ,编码 10 5个氨基酸。比较它们之间的核苷酸及推导氨基酸序列 ,发现有一定差异 ,且抗冷性相差越大的品种间差异越大。抗冷性的差异可能与脯氨酸的替换有关。
Using RT-PCR technique, we obtained a fragment of cDNA about 315bp from rice strains with different chilling-resistance. In amplification reactions, the used primers are degenerated primers, which were designed according to the conserved amino acid sequences of glycerol-3-phosphate acyltransferase from several kinds of dicotyls. Sequences obtained show that the cDNAs fragments all have the parts of cDNAs coding for Glycerol-3-phosphate acyltransferase, contain 315 nucleotides and encode 105 amino acids. While the cDNAs sequences and deduced amino acids sequences from the rice strains with different chilling-resistance were different from each other. The bigger difference of chilling-resistance between strains is, the bigger differences between their cDNAs sequences and the deduced amino acids sequences are. The difference of chilling-resistance is probably related to the subtitution of proline.
出处
《云南植物研究》
CSCD
2000年第3期317-321,共5页
Acta Botanica Yunnanica
基金
国家自然科学基金!(3976 0 0 11)
云南省农科院生物技术所重点实验室基金资助项目
关键词
甘油-3-磷酸转酰酶
cDNA序列比较
抗冷性
水稻
Glycerol-3-phosphate acyltransferase (GPAT)
cDNA sequence comparison
Chilling-resistance
Rice
