摘要
目的：探讨游泳运动对老年大鼠学习记忆能力及伏隔核多巴胺含量和一氧化氮合酶（nNOS）表达的影响。方法：选择24月龄SD老年大鼠，随机分为2组，即老年对照组、游泳运动组，5月龄大鼠为成年对照组。对照组在笼内正常生活，不运动。游泳运动组采用递增负荷游泳运动，连续8周。8周后利用八臂迷宫测试大鼠空间学习记忆能力，高效液相色谱一电化学法检测各组大鼠伏隔核多巴胺含量，采用免疫组织化学结合图像半定量方法对伏隔核nNOS神经元的数量、面积及灰度进行测量和分析。结果：与成年对照组比较，老年对照组大鼠伏隔核多巴胺含量降低，完成八臂迷宫时间延长，工作记忆错误次数、参考记忆错误及总记忆错误次数均增加；与老年对照组比较，游泳运动组大鼠伏隔核多巴胺含量增高，完成八臂迷宫时间缩短，工作记忆错误次数、参考记忆错误次数及总记忆错误次数均减少。与成年对照组比较，老年对照组伏隔核nNOS免疫阳性细胞数量和阳性产物面积均减少，灰度差异没有统计学意义；与老年对照组比较，游泳运动组伏隔核nNOS免疫阳性细胞数量和阳性产物面积均增加，灰度差异无统计学意义。结论：长期游泳运动可提高老年大鼠的学习记忆能力，可能与长期游泳运动有效调整中枢神经递质多巴胺的合成及增强大脑伏隔核nNOS的表达有关。
Objetive.. To explore the influence of the swimming exercise on learning and memory, the concentration of dopa- mine and the expression of nNOS in nucleus accumbens （Nae） of the brain in aging rats. Methods： SD aging rats （24 mouths） were chosen and randomly divided into 2 groups： an aging control group and a swimming exercise group. SD adult rats served as an adult group. The control group kept normal activities and the swimming exercise group undertook an 8-week swimming training with progressively increasing load. Then an 8-arms radial maze was used to assess each animal＇s capability of learning and memory. The concentration of DA was assayed by high performance liquid chromatography coupled with electrochemical detection and the nNOS expression in Nac was detected by using ABC immunohistochemical technique and analyzed semiquan- titatively. Results： Compared with the adult group, the concentration of DA in Nac was decreased, the time of completing 8- arms radial maze was prolonged and working memory error （WME）, reference memory error （RME） and total memory error （TE） were all increased in the aging group; Compared with the aging group, the concentration of DA in Nac increased, the time of completing 8-arms radial maze shortened, WME RME and TE were all decreased in the swimming exercise group; Compared with the adult group, the amount and area of nNOS in Nac were decreased, the gray degree was not significantly different in the aging group; Compared with the aging group, the amount and area of nNOS in Nac were increased; the gray degree increased, with no significant difference in the swimming exercise group. Conclusion： The swimming exercise may en- hance the learning and memory ability of aging rats. Its mechanism might be that the swimming exercise can increase the con- centration of DA and reinforce the expression of nNOS in Nac at the same time.
出处
《解剖学杂志》
CAS
CSCD
北大核心
2013年第2期197-201,共5页
Chinese Journal of Anatomy
基金
中北大学科学基金（2011-2013）
