摘要
目的研究脑络欣通对脑缺血大鼠再灌注损伤的作用机制。方法将40只Wistar大鼠随机分为正常组、模型组、脑络欣通组和通心络组各10只,采用线栓法制作大脑中动脉闭塞模型,2h后拔出栓线少许(约5mm)造成缺血再灌注损伤。脑络欣通组给予浓度为85.8mg/ml脑络欣通溶液1ml/(100g·d),通心络组给予浓度为30mg/ml通心络胶囊溶液1ml/(100g·d),模型组和正常组予以等量生理盐水,各组大鼠连续灌胃14天。采用Morris水迷宫实验检测大鼠在不同时间点逃避潜伏期(EL)及120s内通过原平台所在位置的次数(Visits),分别在造模过程中插线后5min、缺血2h再灌注以及造模后7、14天监测各组大鼠局部脑血流量(rCBF)。结果与正常组比较,模型组和通心络组大鼠在各时间点EL都明显延长,Visits明显减少(P<0.01);与模型组比较,脑络欣通组大鼠各时间点EL显著缩短、Visits显著增加(P<0.01);脑络欣通组大鼠各时间点EL、Visits和通心络组比较差异均有统计学意义(P<0.01)。与正常组比较,其余各组大鼠在各时间点rCBF均低于正常组(P<0.01);与模型组比较,脑络欣通组和通心络组在第7天和第14天大鼠rCBF明显恢复(P<0.01)。结论脑络欣通可能通过增加模型大鼠rCBF而改善学习记忆能力,从而对脑缺血再灌注损伤起到保护作用。
Objective To study the mechanism of Naoluo Xintong Liquid （liquid to free the network vessels of the brain and the heart） for rats with cerebral ischemia-reperfusion injury. Methods Forty Wistar rats were randomized into the normal group, model group, Naoluo Xintong group and Tongxintuo （liquid to free the network vessels of the heart） group, with 10 in each. The middle cerebral artery occlusion rat models were established with suture method and the thread embolism was pulled out a little （about 5 mm） to make ischemia-reperfusion injury 2 hours later. The Naoluo Xintong group and Tongxinluo group was given 1 ml/ （100 g · d） of Naoluo Xintong Liquid （concentration=85.8 mg/ml） and Tongxinluo Liquid （concentration=30 mg/ml） respectively. The model group and normal group were given same amount of normal saline. The aaministration lasted for 14 consecutive days. The Morris water maze test was used to detect the escape [atencies （EL） at different time points and the numbers of passing the location of the original platform within 120 seconds （Visits）. The regional cerebral blood flow （rCBF） was monitored during the modeling, 5 minutes after the thread plugged in, reperfusion after 2 hours of ischemia, 7 days after modeling and 14 days after modeling respectively. Results Comparing with the normal group, the ELs at different time points were significantly prolonged but the Visits were significantly reduced in the model group and Tongxinluo group （P〈0.01）. Comparing with the model group and Tongxinluo group, the ELs at different time points were significantly shortened but the Visits were significantly increased in the Naoluo Xintong group （P%0.01）. Comparing with the normal group,the rCBF at different time points was significantly decreased in other groups （P〈0.01）. Comparing with the model group, the rCBF was restored in the Naoluo Xintong group and Luoxintong group on the 7th and 14th day after modeling （P〈0.01）.Conclusion Naoluo Xintong can improve learning an
出处
《中医杂志》
CSCD
北大核心
2013年第8期687-690,共4页
Journal of Traditional Chinese Medicine
基金
"十二五"国家科技支撑计划资助项目(2012BAI26B00)
安徽省科技厅资助项目(100432)
关键词
脑络欣通
缺血再灌注
水迷宫实验
局部脑血流量
Naoluo Xintong Liquid
ischemia-reperfusiom Morris water maze test
regional cerebral blood flow
