摘要
应用酶消化和机械吹打相结合的方法,快速分离出中华乌塘鳢(Bostrychys sinensis)嗅囊中纤毛嗅觉感受神经元。再应用全细胞膜片钳技术记录中华乌塘鳢纤毛嗅觉感受神经元的静息电位、动作电位及电压门控离子通道电流等电生理学特征。结果表明,中华乌塘鳢纤毛嗅觉感受神经元具有典型的双级神经元结构特征,包括细胞体,轴突,树突以及树突顶端的嗅结和纤毛。嗅觉感受神经元静息电位为(57±6.37)mV,动作电位峰值为(38.30±0.95)mV。在60～+80 mV的阶梯去极化脉冲刺激中,内向电流在60～40 mV激活,20～0 mV达到最大值,内向电流峰值为(69.08±44.15)pA。外向电流在+40 mV时达最大值,峰值为(267.55±73.67)pA。本研究结果为纤毛嗅觉感受神经元上性信息素受体感受性信息素的电生理机制以及性信息素的应用提供了方法和理论基础。
The ciliated olfactory receptor neurons （cORNs） in the olfactory sac were dissociated from Bostrichthys sinensis by enzymatic and mechanical methods. The whole-cell patch-clamp technique was used to determine the resting potential, action potential and voltage-gated ion channel current. The results showed that the B. sinensis cORN was a bipolar neuron, containing cell body, axon and dendrites with olfactory knobs and cilia. The resting and action potential peaks of ciliated olfactory receptor neurons were （-57 ＋ 6.37） mV and （38.30 m 0.95） mV, respec- tively. In response to depolarizing voltage steps pulse stimulation from -60 ±80 mV, the transient inward current was activated between -60 mV and -40 mV, and reached a peak averaging （-69.08 ± 44.15） pA at -20 to 0 mV; the outward current reached a peak averaging （267.55±73.67） pA at ＋40 mV. The present study provides a base theory for research in the electrophysiological mechanisms of sex pheromones with their receptors and the application of sex pheromones.
出处
《中国水产科学》
CAS
CSCD
北大核心
2013年第2期286-292,共7页
Journal of Fishery Sciences of China
基金
国家自然科学基金资助项目(41276129
40776080)
福建省农业科技重大项目(2012N5011)
关键词
中华乌塘鳢
嗅觉感受神经元
膜片钳
电生理学
Bostrichthys sinensis
olfactory receptor neurons
patch clamp
electrophysiology
