摘要
目的:观察复方阿胶浆对小鼠lewis肺癌周期素D1、黏附分子CD44表达的影响,深入探索复方阿胶浆的抗肿瘤机制。方法:建模后,按体重随机分为6组,分别为复方阿胶浆组(中剂量)、环磷酰胺组、复方阿胶浆大、中、小剂量联合环磷酰胺及模型对照组。实验一,取材后以SP免疫组化染色法检测各组肿瘤组织中CyclinD1、CD44的表达水平。实验二,同期观察生存期,连续观察2个月。结果:复方阿胶浆组及复方阿胶浆大剂量联合CTX组能明显下调CyclinD1的表达水平,与模型对照组比较,有统计学意义(P<0.05);除复方阿胶浆小剂量联合CTX组外,余给药组均能下调CD44的表达,与模型组比较有统计学意义(P<0.05);CyclinD1与CD44的表达水平无明显线性相关性(r=0.23221,P>0.05);复方阿胶浆组在一定程度上能够延长小鼠的生存时间。结论:复方阿胶浆的抗肿瘤作用可能与下调肿瘤组织中CyclinD1、CD44的表达水平,干预细胞周期、抑制肿瘤的侵袭性有密切关系,并有可能改善预后。
Objective：To observe the influence of CEJS on CyclinD1 ,and CD44 expression in Lewis lung carcinoma tissues in mice, so as to explore its anti-tumor mechanism. Methods：Murine models were transplanted with LLC cell, which were prepared and divided into a model group and five other groups of CTX, CEJS（ medium dose） , low, medium and high dose CEJS joint CTX prescription. Then the tissues of the six groups were separated for immuno-histoehemical examination of CyclinD1 and CD44. We observed the life period for two months at the same time . Results：CEJS （medium dose） and high dose CEJS joint CTX prescription resulted in more significant suppres- sion of the expression of CyclinD1 （ P 〈 0. 05 ） when compared with the model group. Moreover, the expression of CD44 in every group except the low dose CEJS joint CTX prescription was lower than that in the model group（ P 〈 0. 05 ）. The relation of CyclinD1 and CD44 wasn＇ t approximately linearity（ r = 0. 232 21, P 〉 0. 05 ）. CEJS （ medium dose ） group has a trend to prolong the life period. Conclusion ： Inhibition of the growth of Lewis Lung Carcinoma by CEJS prescription is probably related to its suppressive impact on the expression of CyclinD1 and CD44. It could intervene in the cell cycle, retard the aggression of tumor and maybe improve the prognosis.
出处
《世界中医药》
CAS
2013年第3期318-321,共4页
World Chinese Medicine
