摘要
目的观察长期高盐饮食对Wistar大鼠血压、肾脏的影响及替米沙坦干预作用。方法雄性Wistar大鼠49只,随机分为3组:对照组(n=13,含0.5%NaCl饲料喂养)、高盐组(n=24,含8%NaCl饲料喂养)、干预组(n=12,含8%NaCl饲料喂养+替米沙坦灌胃),均自由饮水,为期24周。尾动脉测压仪监测尾动脉收缩压;代谢笼收集24h尿液;生化方法测定24h微量白蛋白尿(MAU)、总蛋白、肌酐及尿钠、钾含量;24周时,摘取双侧肾脏计算双肾肥大指数(肾质量/体质量),HE、Masson染色观察肾脏形态学改变。结果与对照组比较,高盐组部分大鼠血压持续增高,部分大鼠血压仅在实验第8～10周时出现一过性增高,将其分为高盐高血压组(HH组,n=12)、高盐正常血压组(HN组,n=12)。高盐组大鼠双肾肥大指数,24hMAU、总蛋白、钠排泄量及钠钾比值均增高(P<0.01),内生肌酐清除率(Ccr)降低(P<0.01),肾小管萎缩、坏死,管腔扩张,肾间质纤维化。但与HH组比较,HN组大鼠肾组织损害较轻。干预组大鼠尾动脉收缩压较HH组减低(P<0.01),24hMAU、总蛋白降低(P<0.01),肾小管萎缩、坏死及肾间质纤维化明显减轻,而双肾肥大指数、Ccr、24h尿钠排泄量及钠钾比值差异无统计学意义(P>0.05)。结论长期高盐饮食可引起部分Wistar大鼠血压持续升高,部分Wistar大鼠血压呈一过性增高,且存在独立于血压的肾损害;替米沙坦对其有降压和保护肾脏作用。
Objective To investigate the effect of long-term intaking high-salt diet on blood pressure and kidney function in Wistar rats with or without telmisartan. Methods Forty-nine male Wistar rats were randomly divided into three groups： control group （given 0.5% NaCl, n=13}, High-salt group （given 8% NaCl, n=24）, and intervention group （given 8% NaCl＋telmisartan, n： 12}. All groups had free access to water. The experiment lasted for 24 weeks. Tail systolic blood pressure was monitored by tail-cuff artery pressure-measuring instrument. The 24 h urine was collected by metabolism cage to measure the concentration of Na＋ , K＋ , microalbumin, total protein, and creatinine. At the 24th week, both kidneys were obtained to calculate the renal hypertrophy index {kidney mass/body mass}. The kidney morphological changes were observed by Hematoxylin and Eosin{ HE} staining and Masson staining. Results Compared to control group, blood pressure of some rats in High-salt group was continuously increased,while blood pressure of the others in this group only increased during the 8th to 10th week of the experiment. Thus the rats in High-salt group rats were further divided into High-salt hypertension group { HH group, n= 12} and High-salt normal blood pressure group { HN group, n= 12）. In High-salt group, the renal hy- pertrophy index, 24 h urinal microalbumin, totel protein, sodium excretion and the ratio of Na＋/K＋ were increased {P（0.01}, while the creatinine clearance rate{Ccr} was decreased {P（0.01} with tubular atrophy and necrosis, luminal expansion, and renal interstitial fibrosis. The renal damages in HN group were lighter than those in HH group. Compared to HH group, the tail systolic blood pressure, content of microalbumin and totel protein was decreased {P〈0.01） in intervention group,and renal damages were ameliorated. But the renal hypertrophy index, Ccr, 24 h sodium excretion, and the ratio of Na＋/K＋ didn＇t have statistically significant change （P〉0.05）. Conclusions Long-term hig
出处
《中华高血压杂志》
CAS
CSCD
北大核心
2013年第3期253-259,共7页
Chinese Journal of Hypertension
基金
贵州省社会发展攻关计划项目
省高层次人才科研条件特助项目[黔科合SY字(2011)3047号,TZJF-2009年-42]
国家自然科学基金资助项目(81160041)
