摘要
目的探讨血管基质片段(vascular stromal fraction,SVF)辅助游离脂肪移植促进其早期存活的相关机制。方法取5例行腹部抽脂手术女性患者自愿捐赠的脂肪组织分离、培养SVF。4～6周龄裸鼠24只(体重15～18 g),将裸鼠背部左、右侧随机分为A、B组(n=24),采用Coleman技术分别于A、B组裸鼠背部皮下移植脂肪0.5 mL和含5×105个SVF的脂肪0.5 mL。移植后1、3、5、7 d分别取6只裸鼠,取出移植脂肪组织,行大体观察、CD31免疫组织化学染色及Western blot检测分析。结果随着移植时间增加,A、B组移植脂肪组织湿重均呈上升趋势,但各时间点A、B组间比较差异均无统计学意义(P>0.05)。移植后1、3 d两组均未见CD31阳性细胞;5、7 d B组CD31阳性细胞数显著高于A组(P<0.05),两组7 d时CD31阳性细胞数显著高于5 d时(P<0.05)。Western blot检测示,两组移植脂肪中VEGF蛋白表达在移植后第3天达峰值,随后开始下降;1、3、5 d B组VEGF蛋白表达明显高于A组(P<0.05)。两组移植脂肪中HGF蛋白表达在移植后前5 d均保持在较高水平,7 d开始下降,B组均明显高于A组(P<0.05)。结论在游离脂肪移植早期,SVF通过旁分泌生长因子促进移植脂肪血管生成。
Objective To investigate the mechanism of vascular stromal fraction （SVF） at the early stage after aspirated fat transplantation. Methods Fat was harvested from 5 cases of women undergoing abdominal liposuction operation, and SVF was isolated. Aspirated fat with （group B） or without （group A） SVF was injected subcutaneously into the back of nude mice, and the grafts were harvested at 1, 3, 5, and 7 days. Graft wet weight was measured; and immunohistochemical method （CD31） was performed and the secretion of vascular endothelial growth factor （VEGF） and hepatocyte growth factor （HGF） were qnantified by Western blot assay. Results The wet weight of transplanted adipose tissue showed an increasing tendency in groups A and B with time, and no significant difference was found between groups A and B （P 〉 0.05）. At 1 and 3 days after transplantation, no CD31 positive cells was seen in 2 groups; the CD31 positive cells of group B were significantly more than those of group A at 5 and 7 days （P 〈 0.05）, and the CD31 positive cells at 7 days were significantly more than those at 5 days in 2 groups （P 〈 0.05）. Western blot test showed that VEGF expression reached peak at 3 days, then decreased gradually; the expression of VEGF protein in group B was significantly higher than that in group A at 1, 3, and 5 days （P 〈 0.05）. The expression of HGF protein in groups A and B remained at a high level within 5 days, but it tended to decrease at 7 days, which was significantly higher in group B than that in group A （P 〈 0.05）. Conclusion SVF can enhance angiogenesis by secretion of growth factors at the early stage after aspirated fat transplantation.
出处
《中国修复重建外科杂志》
CAS
CSCD
北大核心
2013年第4期449-453,共5页
Chinese Journal of Reparative and Reconstructive Surgery
基金
国家自然科学基金资助项目(81071589
81171834)
广东省自然科学基金资助项目(S2011010003058
S2012010009370)
南方医院院长基金资助项目(2011C008)~~
关键词
血管基质片段
游离脂肪移植
血管生成
生长因子
裸鼠
Vascular stromal fraction Aspirated fat transplantation Angiogenesis Growthfactor Nude mouse
