摘要
目的:检测人乳头状瘤病毒(HPV)16-E6蛋白在外阴上皮内非瘤样病变(NNEDV)、外阴鳞癌(VSCC)中的表达,探讨HPV16-E6蛋白是否为NNEDV病因及与VSCC的相关性。方法:采用免疫组织化学SP法检测HPV16-E6在15例正常外阴组织,40例NNEDV及45例VSCC中的蛋白表达情况。结果:HPV16-E6蛋白在正常外阴皮肤组无表达,在NNEDV及VSCC中阳性表达率分别为30%和66.67%,差异均有统计学意义(P<0.05)。在NNEDV组中,HPV16-E6蛋白在鳞状上皮增生(SH)型及硬化性苔藓(LS)型阳性率分别为35%和25%,差异无统计学意义(P>0.05),但均较正常外阴皮肤组升高(P<0.05),较VSCC组降低(P<0.05)。HPV16-E6在VSCC的表达阳性率为66.67%,阳性率随临床分期的增高而增高,I期和II期,I期和III期比较差异均有统计学意义(P<0.017),但II期和III期比较差异无统计学意义(P>0.017)。随着肿瘤分化程度的增高,阳性率逐渐降低,高分化和低分化,中分化和低分化比较差异均有统计学意义(P<0.017),但高分化和中分化比较差异无统计学意义(P>0.017)。有淋巴结转移者HPV16-E6阳性表达率高于无淋巴结转移者(P<0.05)。结论:HPV感染可能是NNEDV的病因之一。HPV16-E6蛋白表达升高可能与VSCC发生、发展相关。
Objective： To investigate the expression of high risk human papilloma virus （HPV） 16-E6 protein in non-neoplastic epithelial disorders of the vulva （NNEDV） and squamous cell carcinoma of the vulva （VSCC）, and to explore whether HPV16-E6 protein is the etiological factor in NNEDV and its correlation with squamous cell carcinoma of the vulvae.Methods： We detected HPV16-E6 protein expression in 15 normal vulvae cases, 40 NNEDV cases and 45 VSCC cases by immunohistochemistry SP method. Results： The positive rate of HPVI6-E6 in different vulva tissues： was 0% in the normal vulva, 30% in NNEDV and 66.67% in VSCC, respectively. The overall positive rate and two two comparison had statistical significance. In the NNEDV group, the positive rate of squamous hyperplasia type and lichen sderosus type was 35% and 25%, respectively, with no statistical significance （P〉0.05）, but higher than that in the normal vulva skin group （P〈0.05） and lower than that in the VSCC group （P〈0.05）. The positive rate of HPV16-E6 in VSCC was 66. 67%. The positive rate increased with the clinical stage. The positive rate between Phase Ⅰ and PhaseⅡ, and that between Phase Ⅰ and Phase Ⅲ had statistical significance （P〈0.017）, but that between Phase Ⅱ and Phase Ⅲ had no statistical significance （P〉0.017）. The positive rate gradually decreased with the tumor differentiation. The difference in well-differentiated and poorly differentiated, moderately and poorly differentiated had statistical significance （P〈0.017）, but that of well-differentiated and moderately differentiated had no statistical significance （P〉0.017）. The positive rate of lymph node metastasis VSCC was significantly higher than that of non-lymph node metastasis VSCC （P〈0.05）. Conclusion： HPV infection may be an etiological factor for NNEDV. The rise of HPV16-E6 positive rate may be related to the occurrence and development of vulvar squamous cell carcinoma.
出处
《中南大学学报（医学版）》
CAS
CSCD
北大核心
2013年第3期225-230,共6页
Journal of Central South University ：Medical Science
基金
湖南省自然科学基金(11JJ5055)~~
