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小麦磷脂酶Dα的基因克隆及其编码序列的生物信息学分析 被引量:4

Cloning and Sequence Bioinformatics Analysis of Phospholipase Dα Gene from Wheat
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摘要 为获得更多小麦抗逆基因资源,利用RT-PCR方法克隆了磷脂酶D基因(PLD),命名为TaPLDα,并对基因及其蛋白进行了生物信息学分析。结果表明,TaPLDα开放阅读框为2 394 bp,编码812个氨基酸残基,等电点5.30,分子量为92 kDa。序列分析显示,TaPLDα基因编码的氨基酸序列含有N端C2结构域及2个保守的活性中心。预测TaPLDα蛋白是一个亲水性稳定蛋白且定位于细胞质,其二级结构含28.82%的α-螺旋、20.07%的延伸链、6.03%的β-转角和45.07%的不规则卷曲。该蛋白不存在信号肽,无跨膜区。TaPLDα与水稻、玉米和拟南芥的PLDα氨基酸序列之间具有高度的保守性,进化分析显示,小麦PLDα序列与毒麦、水稻和玉米PLD序列亲缘关系密切。 To obtain more wheat(Triticum aestivum L.)stress-response genes,a phospholipase D gene,named TaPLDα,was cloned by RT-PCR.And the protein of this gene was also predicted and studied through the bioinformatics analysis method.The results showed that the largest open reading frame of TaPLDα gene has 2 394 bp in length and encoded a polypeptide of 812 amino acid residues.The estimated molecular weight and isoelectric point of the putative protein were 92 kDa and 5.30,respectively.Sequence analysis showed that the amino acid sequence encoded by TaPLDα gene contained N-terminal C2 domain and two HKD motifs.TaPLDα protein was a stable hydrophilic protein and located in the cytoplasm.The predicted secondary structure composition for the protein has about 28.82% alpha helixes,20.07% extended strand,6.03% beta turn and 45.07% random coil.The TaPLDα had no signal peptide and transmembrane helices.Compared with rice,maize and Arabidopsis,amino acid sequence encoded by TaPLDα was almost conserved.The phylogenic tree showed that TaPLDα was most similar to PLDα protein from darnel,rice and maize.
出处 《华北农学报》 CSCD 北大核心 2013年第1期117-122,共6页 Acta Agriculturae Boreali-Sinica
基金 国家"863"计划项目(2011AA100104) 天津市应用基础与前沿技术研究计划项目(11JCYBJC09100) 国家转基因新品种培育重大专项(2009ZX08009-084B)
关键词 小麦 磷脂酶Dα 基因克隆 生物信息学 Wheat Phospholipase Dα Gene cloning Bioinformatics
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  • 1Hanahan D J,Chaikoff I L. A new phosphorus-containing lipids of the carrot [ J ]. J Biol Chem, 1947,168 : 233 - 240. 被引量:1
  • 2Wang X,Xu L,Zheng L. Cloning and expression of phos- phatidylcholine-hydrolyzing phospholipase D from Ricinus communis L[ J]. J Biol Chem, 1994,269:20312 - 20317. 被引量:1
  • 3Wang X. The role of phospholipase D in signal transduc- tion cascade [ J]. Plant Physiol, 1999,121:456 - 462. 被引量:1
  • 4Li M, Hong Y, Wang X. Phospholipase D- and phospha- tidic acid-mediated signaling in plants [ J ]. Biochim Bio- phys Acta,2009,1791:927 - 935. 被引量:1
  • 5Qin C,Wang X. The Arabidopsis phospholipase D family: Characterization of a calcium independent and phosphati- dylcholine-selective PLDξ1 with distinct regulatory do- mains[ J]. Plant Physiol,2002,128 : 1057 - 1068. 被引量:1
  • 6Wang C,Wang X. A novel phospholipase D of Arabidopsis that is activated by oleic acid and associated with the plasma membrane [ J ]. Plant Physiol, 2001,127 : 1102 - 1112. 被引量:1
  • 7Wang X. Regulatory functions of phospholipase D and phosphatidic acid in plant growth, development, and stress responses [ J ]. Plant Physiol, 2005, 139 : 566 - 573. 被引量:1
  • 8Hong Y, Zhang W, Wang X. Phospholipase D and phos- phatidic acid signaling in plant response to drought and salinity[J]. Plant Cell Environ,2010,33:627 -635. 被引量:1
  • 9Bargmann B O, Laxah A M, ter Riet B, et al. Multiple PLDs required for high salinity and water deficit tolerance in plants[J]. Plant Cell Physiol,2009 ,50 :78 -89. 被引量:1
  • 10Hong Y,Pan X,Welti R,et al. Phospholipase Dα3 is in- volved in the hyperosmotic response in Arabidopsis [ J ]. Plant Cell ,2008,20:803 - 816. 被引量:1

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