摘要
[目的]对普洱茶渥堆发酵过程中的细菌进行分离、纯化和鉴定。[方法]采用传统培养方法与分子生物学方法相结合,对普洱茶渥堆发酵各阶段的细菌进行了分离、纯化及鉴定。[结果]试验表明,普洱茶渥堆发酵各阶段的细菌包括了芽孢杆菌、克雷伯氏菌、鞘氨醇杆菌、短杆菌、红球菌、假单胞菌、鲍曼不动杆菌7个种类,其中芽孢杆菌所占比例远大于其他细菌,处于绝对优势地位。[结论]研究可为研制普洱茶高效发酵剂、缩短发酵时间、改善普洱茶品质,以及实现普洱茶清洁化和产业化生产提供一定的理论依据。
[Objective] To isolate,purify and identify bacteria during Pu-erh Tea pile fermentation process.[Method] The bacteria colonization was isolated from Pu-erh Tea in the pile fermentation process and investigated by using molecular biological methods.[Result] The results showed that there are 7 species of bacteria including Bacillus,Klebsiella pneumoniae,Sphingomonas bacteria,Brevibacterium,Rhodococcus,Pseudomonas,Acinetobacter baumannii and so on.The proportion of Bacillus cereus which located in a dominant position is much larger than other bacteria.[Conclusion] The study can provide theoretical basis for producing efficient leaven,shortening fermentation time,improving Pu-erh Tea quality,and realizing clean and industrialization production of Pu-erh Tea.
出处
《安徽农业科学》
CAS
2013年第6期2667-2668,2671,共3页
Journal of Anhui Agricultural Sciences
基金
云南农业大学博士启动基金项目(A2002157)
关键词
普洱茶
渥堆发酵
细菌
分子鉴定
Pu-erh Tea
Pile fermentation
Bacterial colonization
Molecular identification
