摘要
目的研究哺乳动物雷帕霉素靶蛋白（mTOR）信号通路相关蛋白mTOR和真核起始因子4E结合蛋白1（4E-BP1）在胃癌组织中的活化情况及其临床意义。方法利用免疫组织化学技术观察38例手术患者胃癌组织中mTOR和4E-BP1的活化情况，采用卡方检验及Kruskal-Wallis检验分析磷酸化mTOR及4E-BP1在癌区、癌旁和正常胃黏膜组织中表达的差异，以及各种临床病理参数间的表达差异。结果磷酸化mTOR在胃癌组织中的阳性表达率高于匹配的癌旁及正常组织[71.1％（27／38）比50.0％（19／38）比44.7％（17／38）；X^2=11.031，P=0.026]，其下游的4E-BP1在胃癌组织中的阳性表达率也高于匹配的癌旁及正常组织[68.4％（26／38）比57.9％（22／38）比28.9％（11／38）；X^2=13.943，P=0.007]。磷酸化mTOR和4E-BP1与肿瘤Lauren＇s分型、浸润分期、分化程度、淋巴结转移及患者年龄等无关，但胃癌组织中活化的4E-BP1在不同大小肿瘤间表达差异有统计学意义（H=3.86，P〈0.05）。结论mTOR信号通路在胃癌发生中存在过度激活，其下游蛋白4e-BP1磷酸化程度在不同大小肿瘤中存在差异。
Objective To investigate the activation and clinical significance of the mammalian target of rapamycin （mTOR） pathway related protein and eukaryotic translation factor 4E-binding protein 1 （4E-BP1） in gastric cancer. Methods The activation of roTOR and 4E-BP1 in gastric cancer tissues of 38 surgical patients were detected by immunohistochemical method. The differences of phosphorylated mTOR and 4E-BP1 expression among cancerous tissues, para-cancerous tissues and normal gastric mucosa tissues and dinicopathological variables were analyzed by Chi square test and Kruskal-Wallis test. Results The positive expression rate of phosphorylated mTOR in the gastric cancerous tissues was significantly higher than that of para-cancerous tissues and normal tissues [71.1% （27/ 38）, 50.0%（19/38） and 44.7% （17/38）, X^2 = 11.031, P= 0.026]. The positive expression rate of downstream protein 4E-BP1 in the gastric cancer tissues was also significantly higher than that of para- cancerous tissues and normal tissues [68.40%（26/38）, 57.9%（22/38） and 28.9%（11/38）,X^2=13.943, P=0.007]. There was no correlation between phosphorylated roTOR and 4E-BP1 expression and tumor Lauren＇s sub-type, infiltration, differentiation degree, lymph node metastasis and patient＇s age. There was statistical significant difference between activated 4E-BP1 expression and tumor size in gastric cancer （H=3.86, P〈0.05）. Conclusions mTOR pathway was over activated in gastric cancer. There was difference between phosphorylation degree of its downstream protein 4E-BP1 and the tumor size.
出处
《中华消化杂志》
CAS
CSCD
北大核心
2013年第3期166-170,共5页
Chinese Journal of Digestion
基金
国家自然科学基金青年科学基金（81001070）
