摘要
采用滤纸片法,以抑菌圈直径为评价标准,以金黄色葡萄球菌和大肠杆菌为指标菌,分别利用水以及一定百分数的乙醇提取箬叶,考察不同箬叶提取液抑菌效果。采用响应面设计优化试验得出水提取箬叶最佳提取工艺条件为:提取温度50℃,液固比10∶1(mL∶g),提取时间3 h,结果表明:此提取条件下箬叶提取液对大肠杆菌和金黄色葡萄球菌,具有较好的抑菌效果。
Us disc diffusion method,and investigate the antimicrobial actives of the extracts from Aspidistra elatior Blume,with Staphylococcus aureus and Escherichia coli as indicative bacteria.The extraction technology was optimized with disc diffusion method.With the response surface methodology test,the results showed that the optimum extracting condition was：extraction temperature 50℃,the ratio of liquid and solid,10：1（mL：g）,extraction time 3h.The extracts of Aspidistra elatior Blume has wide Antibacterial spectrum.The results showed that the extracts of Escherichia coli leaves had the antimicrobial actives.
出处
《武汉工业学院学报》
CAS
2013年第1期35-39,共5页
Journal of Wuhan Polytechnic University
关键词
箬叶
金黄色葡萄球菌
大肠杆菌
抑菌实验
Aspidistra elatior Blume
Staphylococcus aureus
Escherichia coli
Inhibitory Activity
