摘要
目的检测c-FLIP在胃癌组织和胃癌细胞系中的表达,探讨其对胃癌细胞凋亡的影响。方法采用逆转录-聚合酶链反应(RT-PCR)法检测6株胃癌细胞系和32例配对的胃癌组织及癌旁形态学正常组织中c-FLIP的表达;应用Annexin V-FITC/PI双染流式细胞术检测下调c-FLIP表达对细胞凋亡的影响。结果 6个胃癌细胞系中存在c-FLIP的差异表达,其中BGC823高表达c-FLIP基因。在32例胃癌组织中有27例(84.4%)高表达c-FLIP基因,而在32例配对的癌旁形态学正常组织中仅有13例(40.6%)表达且表达水平明显低于胃癌组织。shR-NA干扰c-FLIP表达后明显抑制了细胞的凋亡。结论胃癌组织和细胞中存在c-FLIP的高表达,且其与细胞的凋亡相关,提示c-FLIP可能在抑制胃癌细胞凋亡中发挥重要作用。
Objective To examine the expression of c - FLIP in the gastric cancer and gastric cancer cell lines, and to investigate the roles of this molecules on cell apoptosis. Methods Reverse transcription polymerase chain reaction（ RT -PCR）was used to detect the expression of c - FLIP mRNA in 32 cases of gastric cancer and corresponding non - cancerous tissues and six gastric cancer cell lines. Transfect c - FLIP special shRNA into human gastric cancer cells BGC823 and flow cytometry with annexin V - FITC/PI double staining was employed to detect cell apoptosis. Results The c - FLIP mRNA in gastric cancer was significantly higher than that in non - cancerous tissue（P 〈0.05）and its expression level in six gastric cancer cell lines were discrepancy, BGC823 cell maintained higher expression of c - FLIP. c - FLIP special shRNA can suppress the expression of c - FLIP gene and induce the apoptosis of BGC823 effectively. Conclusion The overexpression of c - FLIP gene in gastric cancer is correlated with cell apoptosis, c - FLIP may play an important role in the inhibition of apoptosis in gastric cancer cells.
出处
《青海医学院学报》
CAS
2013年第1期20-24,共5页
Journal of Qinghai Medical College
基金
青海大学医学院2010年中青年科研基金项目(编号:QY2010-07)
