摘要
本文采用大鼠海马脑片盲法的全细胞记录技术 ,研究了Axon公司pClamp采样软件Clampex中P/N漏减的功能意义和作用机制 ,对P/N漏减脉冲电流的采集以及漏减脉冲的时间、极性、箝位电压、数目、位置等参数的设置进行了详细分析。结果表明 ,在采集电压门控性离子通道电流时 。
Using blind whole cell recording technique on rat hippocampal slices, the function and mechanisms of P/N leak subtraction of Clampex program in pClamp acquisition software were studied. The ways of picking up sub pulse current and properly selecting parameters, including time course, polarization, sub holding potential, numbers and location of P/N sub pulse were analyzed in detail. Our results indicate that voltage gated ion channel currents recorded by Clampex will be more accurate and reproducible by properly using P/N leak subtraction. [WT5HZ]
出处
《生理学报》
CAS
CSCD
北大核心
2000年第5期440-443,共4页
Acta Physiologica Sinica
关键词
漏减
膜片箝
海马脑片
leak subtraction
patch clamp
hippocampal slice
