摘要
目的 探索应用于膜片钳研究的脑片制备方法.方法 配制人工脑脊液以及切片所用的cutting液.将2 d大的幼鼠断头取脑,用振动切片机将脑切成脑组织切片,然后放入孵育槽内孵育备用.在孵育0.5～1 h后在显微镜下观察细胞的形态结构.结果 根据本方法操作,制得的脑组织切片细胞活性较高.结论 脑片可以满足膜片钳实验的需要.
Objective To explore the preparation method of the brain tissue slices applying to patch clamp study. Methods First,the artificial cerebrospinal fluid and cutting solution should be prepared. After decollating a 2-day- old rat,its brain was transferred into a microtome,which cut the brain into slices( 300 μm). Then the brain slices were kept in an incubation chamber for 0. 5 to 1 hour. Finally,the structures of the brain tissue could be observed un- der a microscope. Results The cell activity in the brain tissue with the method above were higher. Conclusion The brain tissue can meet the needs of patch clamp research.
出处
《吉林医药学院学报》
2013年第5期326-328,共3页
Journal of Jilin Medical University
关键词
脑片
膜片钳
孵育槽
brain slice
patch clamp
incubation chamber
