摘要
【目的】建立芒果叶中芒果苷含量的测定方法；测定不同时期芒果叶中芒果苷的含量。【方法】采用高效液相色谱法（HPLC），CosmosilODS柱分离，流动相：乙腈-1％冰醋酸溶液（15：85）为流动相，流速1．0mL／min，柱温25oC，检测波长254nm。从海南采集不同时期的芒果叶，测定其中芒果苷含量。[结果】芒果苷在0．0695N2．2240μg范围内进样量与峰面积呈良好的线性关系，平均回收率96．77％，RSD=I．19％。【结论】所建立的芒果苷含量测定方法灵敏度高，准确度高，重现性好。
Objective] To establish the determination method of Mangiferin content in mango tree leaves for analyzing samples collected in different season. [Methods] HPLC method was performed on Cosmosil ODS column. Mobile phase： CHaCN-I% HAe （15：85）, flow rate： 1 mL/min, column temperature： 25 ℃, detection wavelength： 254 nm. The samples of mango tree leaves were collected in different seasonin from Hainan province for determination of mangiferin. [Results] The method showed a linear relationship between peak area and injection amount at the range of 0.069 5-2.224 0 μg for mangiferin. The average recovery rate was 96.77%, RSD=l.19%. [Conclusion] This HPLC method for the content determination of mangiferin shows good sensitivity, accuracy and repeatability.
出处
《天津中医药大学学报》
CAS
2013年第1期43-45,共3页
Journal of Tianjin University of Traditional Chinese Medicine
基金
国家自然科学基金项目(81173524)
国家科技重大专项项目-重大新药创新(2012X09103201031)
天津市科技创新体系及条件平台建设计划项目(10SYSYJC289000)
教育部新世纪优秀人才计划项目(NCET-10-0958)
