摘要
目的:优选骨愈搽剂的提取工艺。方法:以羟基红花黄色素A含量为指标,采用HPLC测定,通过L9(34)正交试验法考察乙醇体积分数、提取次数、溶剂用量、提取时间对骨愈搽剂提取工艺的影响。结果:最佳提取工艺为加12倍量70%乙醇回流提取2次,每次1.5 h。结论:优选的提取工艺稳定可行,且羟基红花黄色素A含量较高。
Objective： To optimize extraction process of Guyu liniment. Method： Orthogonal test was employed to optimize extraction procedure, including the concentration of ethanol, the amount of solvent, extraction time and extracting times. The content of hydroxy safflor yellow A （HSYA） was used as index which was determined by HPLC. Result： Optimum extracting process for Guyu liniment was as follows： reflux extracted 2 times with 12 times the amount of 70% ethanol, 1.5 h per time. Conclusion： This optimized process was stable and feasible with relatively high content of HSYA.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第7期39-41,共3页
Chinese Journal of Experimental Traditional Medical Formulae
基金
北京市中医药科技项目(JJ2011-36)
