摘要
目的:研究羟基红花黄色素A(羟A)在大鼠体内的吸收机制。方法:大鼠灌胃给药和大鼠胃肠道不同部位分别给药后,用HPLC测定羟A的血药浓度和胃肠道中羟A残留量;大鼠小肠推进实验。结果:橄榄油、维拉帕米和环孢素显著性促进羟A的吸收(P<0.01);橄榄油和川芎挥发油延缓了药物在肠道内的推进速度(P<0.01);羟A在胃内不吸收;羟A在肠道内的吸收程度从高到低依次为:空肠、十二指肠、回肠;羟A在胃肠道内的稳定性从大到小依次为:回肠、胃、空肠、十二指肠。结论:大鼠口服羟A时生物利用度受胃肠道蠕动速度、P-糖蛋白、吸收部位及其在胃肠道内稳定性的影响。
Aim：To study the absorption mechanism of hydroxysafflor yellow A （Hya） in rat stomach and small intestinal segments. Methods： Hya absorption was investigated in rats after ig administration and injection into duodenum,jejunum or ileum. Hya plasma concentrations were measured by HPLC. Intestinal transmit experiments were performed in rats. Results：Olive oil, verapamil and cyclosporin A all showed the absorption enhancing effect on Hya （P 〈 0.01 ）. Olive oil and volatile oils of rhizoma chuanxiong delayed the intestinal transmit of rat （P 〈 0.01 ）. Hya was not absorbed in rat stomach. The absorption extend of Hya in rat intestine was ranked in order of jejunum, duodenum and ileum. In addition, the stability of Hya in rat stomach and intestinal segments was placed in the order of ileum, stomach, jejunum and duodenum. Conclusion：These results indicate that the bioavailability of Hya in rat can be affected by the intestinal transmit, P-glycoprotein, gastrointestinal location and stability in vivo.
出处
《中国药科大学学报》
CAS
CSCD
北大核心
2006年第4期312-317,共6页
Journal of China Pharmaceutical University
基金
国家自然科学基金资助项目(No.30430790)
江苏省自然科学基金资助项目(No.BK2004419)~~
关键词
羟基红花黄色素A
吸收机制
肠蠕动
P-糖蛋白
稳定性
hydroxysafflor yellow A
absorption mechanism
intestine transmit
P-glycoprotein
stability in vivo
