摘要
目的:对小儿咽扁颗粒剂的制备工艺和质量标准分别进行优化与深化。方法:采用正交试验对水提工艺条件进行优化;采用薄层色谱法对金银花、射干、冰片、人工牛黄分别进行定性鉴别;采用高效液相色谱法对金银花中绿原酸进行定量分析,色谱柱为C18Diamonsi(l200 mm×4.6 mm,5μm),流动相为乙腈-0.1%磷酸溶液(13∶87),流速为1.0 mL.min-1,检测波长为327 nm。结果:制备工艺简便可行;薄层色谱斑点清晰,专属性强;绿原酸进样量在0.0411-0.3699μg范围内线性关系良好(r=0.99996),平均回收率为98.31%,RSD%为0.71%。结论:所建立的定性定量方法简便、准确、重复性好,可作为小儿咽扁颗粒剂的质量控制方法。
Objective： To optimize and deepen the preparative technique and quality standard of Xiao＇er Yanbian granules. Methods： The orthogonal test was adopted to optimize the extraction process in water. Flos Lonicerae, Rhizoma Belamcandae, Borneolum Syntheticum and Bovis Calculus Artifactus were identified by TLC; and the contents of chlorogenic acid in Flos Lonicerae were determined simultaneously by HPLC.The chromatographic column was Diamonsil C18 column（200 mm×4.6 mm, 5 μm）, the mobile phase was acetonitrile-0.1% phosphoric solution（13：87） at the flow rate of 1.0 mL＇min-l,and the detecting wave- length was 327 nm. Results： The preparative technique is simple and feasible. The TLC identification was highly specific and spots were clear. Chlorogenic acid showed a good linear relationship at a range of 0.0411-0.3699 μg（r=0.99996）, and the aver- age recovery was 98.31% with RSD of 0.71%. Conclusion： The qualitive and quantitative methods established is simple, accu- rate and reproducible, and can be used for the quality control of Xiao＇ er Yanbian granules.
出处
《中医药导报》
2013年第3期82-84,共3页
Guiding Journal of Traditional Chinese Medicine and Pharmacy
关键词
小儿咽扁颗粒剂
绿原酸
工艺优化
定性定量
Xiao＇er Yanbian granules
Chlorogenic acid
Technology optimization
Quality and quantity
