摘要
目的改良羊水染色体制备技术。方法与传统羊水细胞染色体制备方法不同,在常规预固定加2次甲醇和冰醋酸3∶1(V∶V)固定基础上,新增1次甲醇和冰醋酸1∶3反比例固定操作,随机选取194个羊水标本制备染色体,比较新旧方法在制片成功率、染色体分散度和显带方面的差异。并应用改良方法对3 726例羊水标本进行核型分析。结果与传统方法相比,增加反固定方法不仅能显著提高羊水培养成功率(P<0.05),而且获得核型分散效果更好、染色体分辨率更高(P<0.05)。3 726例羊水标本中,一次性培养成功3 671例,成功率为98.52%,检出异常核型265例,异常率为7.22%。结论羊水染色体制备改良方法中增加反固定方法,所获核型好、成功率高,便于临床推广。
Objective To improve the protocol for chromosome preparation from anmiotic fluid cells. Methods An additional fixation was applied using 1 ： 3 （V ： V ） of methanol and glacial acetic acid based on the conventional pre-fix plus two times of fixation [ 3 ： 1 （ V ： V ） of methanol and glacial acetic acid 1;194 cases were randomly selected to compare successful rate,chromosome dispersion and G-banding be- tween the old and new methods. Prenatal diagnosis was carried out in 3 726 arrmiotic fluid samples using the improved procedure. Results Statistical analysis data indicated that the new method improved the successful rate of chromosome analysis, and achieved better karyotypes （P 〈 0.05 ） compared with traditional method. 3 671 cases were cultured successfully in 3 726 cases with the successful rate of 98.52%, and ＇abnormal karyotype were found in 265 fetuses （7.22%）. Conclusion We established a novel and effective method of chromosome prepa- ration with high suceess rate, better splitting phase, and ease of operation with anmiotie fluid cells.
出处
《中国医科大学学报》
CAS
CSCD
北大核心
2013年第3期274-276,共3页
Journal of China Medical University
基金
国家自然科学基金资助项目(81202046)
关键词
羊水细胞培养
核型分析
产前诊断
amniotic fluid cell culure
karyotyping
prenatal diagnosis
