摘要
目的调查2型糖尿病患者群唾液中牙周致病菌的感染情况，初探血糖异常对口腔内牙周致病菌定植生长的影响。方法筛选北京石景山社区的高血压人群，留取其中的45名非糖尿病者（非DM组）、80例血糖控制稳定（DM-好组）和100例血糖控制差的2型糖尿病患者（DM-差组）的静息全唾液，提取唾液中细菌基因组DNA，应用以16SrRNA为基础的PCR技术检测牙龈卟啉单胞菌（Porphyromonas gingivalis，Pg）、福赛坦菌（Tannerella forsythia，Tf）和齿垢密螺旋体（Treponema denticola，Td）3种牙周致病菌。应用SPSS15．0统计软件对不同血糖状况下唾液中3种牙周致病菌的检出差异进行比较，并在Logistic回归模型基础上分析与各种菌检出相关的变量因素。结果DM-好组和DM-差组的Tf检出率[分别为81％（65／80）和80％（80／100）]均显著低于非DM组[91％（41／45）]，差异均有统计学意义（P〈0．05），DM-好组和DM-差组的Tf相对含量[分别为1．9（2．6）、2．1（5．3）]均显著低于非DM组[3．4（6．4）]（P〈0．05），且血糖控制越差，Tf的相对含量越低。Pg、Td在3组受检者中的检出率和相对含量差异均无统计学意义（P〉0．05）。调整了年龄、性别、缺牙数和其他牙周临床指标，DM-好组和DM-差组唾液中Tf定植的风险均较非DM组低，OR值分别为0．58和0．53。结论Pg、Td在口腔内的定植可能未受血糖影响，血糖异常可能会影响口腔内Tf的定植。
Objective To investigate the prevalence of periodontal pathogens from saliva of patients with type 2 diabetes melliius （ T2DM）, and to characterize the association between the glucose status and periodontal pathogens in oral cavity. Methods All the subjects were hypertension patients under regular care at Beijing hypertension prevention and management institute. Whole unstimulated saliva samples were collected from 45 non-diabetic subjects （non-DM group ）, 80 well-controlled diabetic patients （DM-well group） and 100 poor-controlled diabetic patients （DM-poor group）. DNA was extracted from the salivary deposition, Porphyromonas gingivalis （ Pg ）, Tannerella forsythia （Tf） and Treponema denticola （ Td ） were detected by polymerase chain reaction（PCR） method based on 16SrRNA. Prevalence and quantity of the pathogens under different glucose states were compared and logistic regression model was set to analyze the factors related to each bacterium. Results The prevalence of Tf in DM-well group and DM-poor group was significantly lower than that of non-DM group [ 81% （ 65/80 ）, 80% （ 80/100 ） vs 91% （41/45）, P = 0. 048 ], meanwhile the quantity of Tf was also lower than that of non-DM group [ 1.9 （2. 6）, 2. 1 （ 5.3 ） vs 3.4 （6.4） ] （ P 〉 0. 05 ）. With the worsening of glucose control, the quantity of Tf was declining （ P = 0. 032）. However, the prevalence and the quantity of Pg, Td in 3 groups had no statistical differences（P 〉 0. 05）. After adjusting age, gender, number of missing teeth and other periodontal parameters, OR of having Tf in saliva from DM-well group and DM-poor group was 0. 58 and 0. 53, respectively. Conclusions Abnormalblood glucose state may affect the colonization of Tf in oral cavity.
出处
《中华口腔医学杂志》
CAS
CSCD
北大核心
2013年第3期144-149,共6页
Chinese Journal of Stomatology
基金
“十一五”国家科技支撑计划（2007BA118802）
卫生部部属（管）医院2010--2012年度临床学科重点项目
关键词
糖尿病
2型
牙周炎
唾液
牙周致病菌
Diabetes mellitus,type 2
Periodontitis
Sliva
Periodontal pathogen
