摘要
目的研究 p16和CD15在结肠癌组织中的表达与结肠癌侵袭和转移行为的关系及意义。方法应用免疫组织化学法 (SP法 )检测 10例慢性结肠炎粘膜组织 (对照组 )和 31例结肠癌组织中p16及CD15的表达。结果31例结肠癌p16阳性表达率 (4 1% ) ,明显低于对照组 (90 % ) ;CD15阳性表达率为 6 7.74% ,明显高于对照组 (4 0 % ) ,差异均有显著性 (P <0 .0 5 )。 31例结肠癌中 ,p16表达在侵犯和转移者 (B组 )为 31.5 8%低于无侵犯无转移者 (A组 )的 5 0 % ,而CD15表达则高于无侵犯无转移者 (A组 ) (73 .6 8vs 5 8.33) ,差异均有显著性 (P <0 .0 5 )。结论p16低表达和CD15高表达与结肠癌的侵袭和转移密切相关 ;术前检测p16、CD15在结肠癌活检组织中的表达 ,对估计结肠癌侵袭和转移情况 ,指导手术决策 ,以及估计预后有重要的临床应用价值。
Objects To study the relationship between the expression of p16,CD 15 and invasiveness and Metastasis of colon cancer as well as its clinical significance. Methods Using immunohistochemical method (SP method), p16 and CD 15 expressions were detected in ten cases of chronic colonitis (control group) and 31 cases of colon cancer, including 12 cases without invasion and metastasis (Group A) and 19 cases with invasion and metastasis (Group B). Results The positive expression rate of p16 in 31 cases of colon cancer was 41%, evidently lower than that of control group (90%)(P<0.05); while their positive expression rate of CD 15 was 67.74%, evidently higher than that of control group (40%)(P<0.05). Among 31 cases, positive expression rate of p16 in Group B was 31.58%, significantly lower than that of Group A (50%)(P<0.05); whereas the positive expression rate of CD 15 in Group B was 73.68%, significantly higher than that in Group A (58.33%)(P<0.05). Conclusions Low expression of p16 and high expression of CD 15 are closely correlated with invasion and metastasis of colon cancer. Detection of p16 and CD 15 expression in biopsied tissues of colon cancer before operation shows important value of clinical application in the prediction of its invasiveness and metastasis, in guidance upon decision of operation and appraisal of its prognosis. [
出处
《湖南医学》
2000年第5期334-335,共2页
Hunan Medical Journal
