摘要
本试验旨在研究方斑东风螺(Babylonia areolate)对饲料锌的需要量。以硫酸锌为锌源,在以酪蛋白、明胶为主要蛋白质源的基础饲料中分别添加0、5、10、20、30、50mg/kg的锌,使6种试验饲料中锌含量分别达到7.1、10.1、15.3、23.4、33.0、54.4mg/kg。每种饲料饲喂1组方斑东风螺,每组3个重复,每个重复80只螺,螺初始体重为(106.25±0.00)mg。试验期8周,养殖水体中锌含量为5.74μg/L。结果表明:1)饲料锌含量对方斑东风螺饲料系数(FCR)、蛋白质效率(PER)、成活率(SR)和肉壳比(SB/SR)无显著影响(P>0.05)。方斑东风螺增重率(WGR)先随饲料锌含量的升高而显著增加(P<0.05),当饲料锌含量达到15.3mg/kg后各组WGR无显著差异(P>0.05)。2)饲料锌含量对方斑东风螺软体部水分、粗蛋白质、粗脂肪和灰分含量无显著影响(P>0.05)。3)方斑东风螺软体部锌含量与饲料锌含量呈正相关关系(P<0.05,R2=0.975 0),各组方斑东风螺壳中锌含量无显著差异(P>0.05)。4)方斑东风螺内脏团中总超氧化物歧化酶(T-SOD)、铜锌超氧化物歧化酶(CuZn-SOD)、过氧化氢酶(CAT)和碱性磷酸酶(AKP)活力先随饲料锌含量的升高而显著增加(P<0.05),当饲料锌含量达到23.4mg/kg后上述酶活力各组间均无显著差异(P>0.05)。以WGR为指标,通过折线模型分析得出方斑东风螺对饲料锌的最适需要量为15.3mg/kg;以内脏团中T-SOD、CuZn-SOD、CAT和AKP活力为指标,通过折线模型分析得出方斑东风螺对饲料锌的最适需要量为22.9～24.3mg/kg。
This experiment was conducted to determine the dietary zinc （Zn） requirement of spotted babylon, Babylonia areolate. Six doses of 0, 5, 10, 20, 30 and 50 mg/kg Zn from zinc sulfate were supplemented in a basal diet （ casein and gelatin were used as protein sources） to prepare 6 experimental diets. In the 6 experimental diets, the Zn levels were 7.1, 10.1, 15.3,23.4, 33.0 and 54.4 mg/kg, respectively. Each diet was fed one group （ each group had 3 replicates and each replicate had 80 spotted babylon） of spotted babylon with an initial body weight of （ 106. 25 ± 0. 00 ） mg for 8 weeks. The Zn content in rearing seawater was 5.74 μg/L. The results showed as follows ： the survival rate （ SR）, feed conversion rate （ FCR）, protein efficiency rate （PER） and soft body to shell ratio （SB/SR） were not significantly affected by dietary Zn content （ P 〉 0.05 ）. With the increase of dietary Zn content, the weight gain rate （WGR） was significantly increased firstly （P 〈 0.05 ）, and then there was no significant difference among all groups when dietary Zn content reached 15.3 mg/kg （P 〉0.05 ）. 2 ） No significant differences were found in the contents of moisture, crude protein, ether extract and ash in soft body of spotted babylon among all groups （ P 〉 0.05 ）. 3 ） The soft body Zn content of spotted babylon had a positive correlation with dietary Zn content （ P 〈 0.05, R2 = 0. 975 0 ）, but the shell Zn content was not significantly affected by dietary Zn content （ P 〉 0.05 ）. 4 ） With the increase of dietary Zn content, the activities of total superoxide dismutase （ T-SOD）, copper-zinc superoxide dismutase （ CuZn-SOD）, catalase （CAT） and alkaline phosphatase （AKP） in visceral mass of spotted babylon were sig- nificantly increased firstly （ P 〈 0.05 ）, and then no significant differences were found in above enzymic activities among all groups when dietary Zn content reached 23.4 mg/kg （ P 〉 0.05 ）. Using the broken-line
出处
《动物营养学报》
CAS
CSCD
北大核心
2013年第3期643-650,共8页
CHINESE JOURNAL OF ANIMAL NUTRITION
基金
广东省自然科学基金(10152408801000024)
广东省科技计划项目(2009B020309008)
广东省高等职业院校珠江学者岗位计划资助项目(2011)
关键词
方斑东风螺
锌
需要量
spotted babylon
zinc
requirement
