摘要
目的建立一种快速、灵敏、准确的HPLC法测定人血浆中伏立康唑浓度,用于临床上该药的血药浓度监测。方法色谱柱:Agilent Eclipse XDB-C18(4.6 mm×150 mm,5μm),流动相:乙腈-水(含0.1%甲酸)(40∶60),流速:1.0ml.min-1,紫外检测波长:256 nm,内标:醋酸可的松,柱温:40℃,进样量:40μl。结果伏立康唑和醋酸可的松的保留时间分别为5.2 min和6.7 min;血浆中伏立康唑线性范围为0.1～10μg.ml-1(r=0.9999),定量下限为0.1μg.ml-1,日内、日间RSD分别<2.07%、4.20%,低、中、高3个浓度提取回收率分别为82.62%、84.26%和78.18%。结论本方法操作简便,结果准确,适用于临床伏立康唑的血药浓度监测。
Objective To establish a quick, sensitive and accurate HPLC method for the determination of voriconazole levels in human plasma. Methods Chromatography was performed on Agilent Eclipse XDB-C18 column （4.6 mm × 150 mm,5 μm）, with acetonitrile-water （0.1% formic acid） （40： 60） as the mobile phase. The flow rate was 1.0 m] .min-1 and the detection wavelength was 256 nm. Cortisone acetate was used as the internal stand- ard. The column temperature was 40 ℃ and injection volume was 40 μl. Results Voriconazole and the internal standard were eluted at 5.2 min and 6.7 rain, respectively. The calibration curve was linear over the concentration range of 0.1-10 μg -ml - 1 （ r = 0. 9999） with the lower limit of quantitation （LLOQ） at 0.1 txg -ml -i. Intra- and inter-day RSD was below 2.07% and 4.20%, respectively. The extraction recovery of the low, medium and high concentration was 82.62% ,84.26% and 78.18%, respectively. Conclusion The HPLC method for determining the voriconazole level in plasma is simple, highly selective and sensitive.
出处
《解放军药学学报》
CAS
2013年第1期32-34,共3页
Pharmaceutical Journal of Chinese People's Liberation Army
