摘要
【目的】研究缺氧缺血(HI)对新生大鼠脑白质细胞血管内皮生长因子(VEGF)及诱导型一氧化氮合酶(iNOS)表达的影响,探讨VEGF及iNOS在新生大鼠脑白质损伤(WMD)发病机制中的作用。【方法】将3日龄大鼠随机分为实验组及假手术组,建立新生大鼠WMD模型,分别予HI后12、24、48、72h及7d处死,对其脑组织取材后行HE染色观察其病理改变,并应用免疫组织化学法检测其VEGF及iNOS的表达水平。【结果】在新生大鼠脑白质中,1)VEGF表达水平予HI后12h开始明显上升,24h后达高峰,至7d恢复正常,与对照组比较,实验组脑室周围白质VEGF的表达在12、24、48、72h有统计学意义。2)实验组iNOS于HI后12h表达开始增加,72h达高峰,7d仍有表达,与对照组比较,差异有统计学意义。【结论】HI可导致新生大鼠脑白质VEGF及iNOS的表达水平显著增高,参与新生大鼠WMD的病理生理过程。
[Objective] To observe the expressions of vascular endothelial growth factor （VEGF） and inducible nitric oxide synthase（iNOS） protein in the brains of premature rats to moderate hypoxia-ischemia （HI）,in order to explore possi-ble relationships with white matter damage（WMD）. [Methods] The 3 days old rats were randomly divided into experi-ment group and sham operation group. The pups were perfused at 12,24,48,72 h and 7 d of recovery from HI. Immunohis-tochemical technical was applied to investigate the changes of expressions of VEGF and iNOS in periventricular rats white matter tissues. [Results] In the experiment group,the expression of VEGF in periventricular rats white matter started to increase at 12 h and reached the peak at 24 h. The expression of iNOS was up-regulated at 12 h and peaked at 72 h after HI. [Conclusion] HI could induce the prominent increase in the expressions of VEGF and iNOS in white matter of the neo-natal rats＇ brain,and may be involved in the pathophysiological process in the WMD.
出处
《中国儿童保健杂志》
CAS
北大核心
2013年第3期276-279,284,共5页
Chinese Journal of Child Health Care
基金
河南省卫生厅资助课题(200804053)
关键词
脑白质损伤
缺氧缺血
血管内皮生长因子
诱导型一氧化氮合酶
新生大鼠
white matter damage
hypoxia-ischemia
vascular endothelial growth factor
inducible nitric oxide syn-thase
neonatal rat
