摘要
目的:探讨黄连、五倍子醇提物对罗非鱼源性链球菌抑制活性。方法:采用BHI(脑心浸液肉汤)固体培养基琼脂稀释法分别检测黄连、五倍子醇提物对链球菌的最小抑菌浓度(MIC)和最小杀菌浓度(MBC);选取五倍子生药质量浓度MIC(3.125 g.L-1)为该实验的低浓度、2倍MIC为中质量浓度(6.25 g.L-1)、3倍MIC为高质量浓度(9.375 g.L-1)。黄连生药质量浓度MIC(13.33 g.L-1)为低浓度、MBC(20.00 g.L-1)为中浓度、2倍MIC(26.67 g.L-1)为高浓度。挑取链球菌单菌落于各组中,搅拌后,于37℃200 r.min-1的条件下培养,于0.5,1.5,2.5,3.5,4.5,5.5,6.5,7.5,8.5 h取样,计算菌落数,并绘制体外杀菌曲线,观察低、中、高浓度下黄连和五倍子醇提物链球菌的动态杀菌过程。结果:五倍子醇提物低浓度和中浓度、高浓度分别在2.5,0.5 h出现抑菌作用。高、中、低浓度分别在6.5,8.5,8.5 h达到杀菌效果。黄连醇提物各浓度在0.5 h出现抑菌作用。高、中,低浓度分别在2.5,4.5,4.5 h达到杀菌效果。结论:黄连和五倍子均有较强的体外抑菌杀菌作用,特别是黄连,使无乳链球菌无正常生长周期,直接进入衰竭期,达到杀菌效果。
Objective： To study the antifungal effect and antifungal mechanism of the essential ethanol extract of Coptidis Rhizoma and Galla Chinensis on Streptococcus agalactiae from viscera of ill tilapia. Method： Low concentration and middle concentration were determined by the agar dilution method（Brain Heart Infusion Broth）.Select Galla Chinensis MIC（3.125 g·L-1）, 2 time MIC（6.25 g·L-1）, 3 time MIC（9.375 g·L-1） for the experiment concentration.Select Coptidis Rhizoma MIC（13.33 g·L-1）, MBC（20.00 g·L-1）, 2 time MIC（26.67 g·L-1） for the experiment concentration.The single colony was selected into medium, cultured on the 37 ℃ 200 r·min-1, under the three concentrations, on the 0.5, 1.5, 2.5, 3.5, 4.5, 5.5, 6.5, 7.5, 8.5 h samples were collected and the quantity of the bacterial colony was counted.Then, time-kill curves observation of the S. agalactiae was carried out. Result： The antifungal effcet of Galla Chinensis low concentration on occuerred from 2.5 h, until 8.5 h.The antifungal effcet of middle concentration and high concentration from 2.5 h, until 6.5 h.The antifungal effcet of Coptidis Rhizoma, middle concentration and high concentration was found at 2.5 h, low concentration at 4.5 h. Conclusion： The Coptidis Rhizoma and Galla Chinensihas powerful antifungal effcet.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第6期234-237,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
广东省科学技术厅项目(2011B090400543)
"十二五"国家科技支撑计划课题(2011BAI01B01)
