摘要
目的:探讨萎胃康及其拆方治疗慢性萎缩性胃炎(CAG)的可能机制。方法:70只Wistar大鼠随机抽取12只为正常对照组(正常组),其余58只采用多重刺激6周复制大鼠CAG模型。确定造模成功的50只随机分为5组,即模型组、萎胃康全方组(全方组)、拆方I号组(补益组)、拆方II号组(祛邪组)、西药维霉素对照组(西药组),分别ig 0.9%生理盐水(10mL.kg-1)、萎胃康水煎液(8 g.kg-1)、拆方I号水煎液(5.5 g.kg-1)、拆方II号水煎液(2.5 g.kg-1)和维霉素混悬液(0.3 g.kg-1),1次/d。给药30 d后,观察对大鼠胃黏膜组织形态及胃黏膜促胃液素细胞(G细胞)、人生长抑素细胞(D细胞)的影响。结果:全方组大鼠胃黏膜组织学接近正常组。模型组G,D细胞数分别为(24.46±3.39),(14.66±1.57)个/mm2,较正常组G,D细胞数(63.54±6.73),(38.32±3.87)个/mm2明显降低(P<0.01)。与模型组比较,各用药组大鼠胃黏膜G,D细胞数显著升高(P<0.01);与全方组G细胞数(56.66±5.70)个/mm2,D细胞数(34.72±4.01)个/mm2比较,补益组、祛邪组、西药组大鼠胃黏膜G,D细胞数显著降低(P<0.01或P<0.05)。结论:萎胃康能改善和逆转实验性萎缩性胃炎大鼠胃黏膜萎缩,其机制可能与改善胃肠激素对胃功能的调节作用有关。
Objective： To study the possible mechanism of Weiweikang （WWK） on treating chronic atrophic gastritis（CAG）. Method： Randomly selected 12 rats from 70 wistar rats were as normal control group（normal group）.The remaining 58 rats were made CAG model with the multiple stimulations for 6 weeks.The remaining 50 rats which were modeled successfully were randomly divided into five groups such as model group, WWK whole formula group （the whole formula group）, its component group （Buyi group）, component II group （Quxie group） and western medicine control group （western medicine group）.The groups were ig 0.9% saline（0.09 g·kg-1）, WWK decoction（8 g·kg-1）, component I（5.5 g·kg-1）, components II（2.5 g·kg-1） and the neomycin-dimensional suspension （0.3 g·kg-1）, once a day.After 90 days, the gastric mucosa pathological changes were observed, the change of G and D cell in gastric mucosa was detected. Result： There was no marked difference in gastric mucosa pathematology between the whole formula group and the normal group.Compared with normal control group whose G cell was （63.54±6.73） cell/mm2 and D cell was （38.32±3.87） cell/mm2, G cell of model group with （24.46±3.39） cell/mm2 and D cell of model group with （14.66±1.57） cell/mm2 decreased significantly（P〈0.01）.Compared with model group, the other medication groups had a significant increase of G and D cell（P〈0.01）;Compared with the whole formula group with G cell： （56.66±5.70） cell/mm2, D cell： （34.72±4.01） cell/mm2, the Buyi group, Quxie group and neomycin-dimensional suspension group decreased significantly （P〈0.01 or P〈0.05）. Conclusion： WWK can significantly repair gastric mucosa in rats with CAG and its mechanism may be actualized by improving the gastrointestinal hormone＇s regulating effects on stomach.
出处
《中国实验方剂学杂志》
CAS
北大核心
2013年第6期199-202,共4页
Chinese Journal of Experimental Traditional Medical Formulae
基金
国家自然科学基金项目(81072782)
山东省科技发展计划项目(2011YD19012)
滨州市科技发展计划项目(2011ZC0911)
关键词
萎胃康
拆方
萎缩性胃炎
G细胞
D细胞
Weiweikang
analysis of the prescription
chronic atrophic gastritis
G cell
D cell
