摘要
出生24小时的乳地鼠,经腹腔(ip)、肌肉(im)及皮下(sc)接种流行性出血热(EHF)病毒A-16株,均可引起系统性感染,导致急性发病死亡。皮下接种组的存活时间比腹腔和肌肉注入者稍长。接种病毒时的鼠龄与死亡率相关,3日龄的乳地鼠感染后全部死亡,9日龄乳地鼠的病死率则为50％。甲免疫组化法检查病毒分布表明,全身各器官中均存在特异性病毒抗原,其中尤以脑、肺、肾的病毒抗原量最多。各器官的病理变化基本上与EHF病毒抗原的组织定位一致。
We observed that newborn outbred suckling hamsters are uniformly susceptible to epidemic hemorrhagic fever ( EHF ) virus infection. With an inoculum of 0.05 ml of 10% virus suspension ip., the clinical course, mean time to death and fatality rate were age-dependent. Mortality was 100% in suckling hamsters infected within 72hr of birth, but declined to 50% by 9 days. Specific viral antigen were detected in all organs examined with highest titers in the brain, lung and kidney both by immunofluorescence and immunoperoxidase assays. Pathological study demonstrated hemorrhage, degeneration and necrosis of parenchymal tissues of various organs, especially in the brain, and kidney. Based on these findings, we conclude that the pathological changes in organs were associated with the distribution of viral antigen.
出处
《病毒学报》
CAS
CSCD
北大核心
1991年第3期231-234,共4页
Chinese Journal of Virology
关键词
流行性出血热
病毒
乳地鼠
Suckling hamster Epidemic hemorrhagic fever virusAnimal model
