摘要
目的探讨刺槐素在SH—SY5Y细胞氧-葡萄糖剥夺和小鼠脑缺血再灌注中的神经保护作用。方法培养神经母细胞瘤细胞SH—SY5Y，分为正常培养组、氧-葡萄糖剥夺（oxygen—glucosedeprivation，OGD）组以及刺槐素小剂量组（1μmol／L）、中剂量组（5μmol／L）和大剂量组（10μmol／L），复氧24h后应用噻唑蓝染色法测定细胞存活率，乳酸脱氢酶（1actatedehydrogaase，LDH）法测定LDH漏出率。60只C57小鼠随机分为假手术组、脑缺血再灌注组以及小剂量组、中剂量组和大剂量刺槐素组，每组12只。采用线栓法制作大脑中动脉闭塞模型，缺血再灌注时给予刺槐素腹腔注射（小、中、大剂量组分别为6．25、12．5和25mg／kg）。再灌注后24h时进行神经功能评分，应用2,3，5-氯化三苯基四氮唑染色检测脑梗死体积。结果体外实验显示，1mol／L、5μmol／L和10μmol／L-1]槐素组SH—SY5Y细胞存活率分别为（90．34±6．87）％（P=0．000）、（85．47±2．24）％（P=0．001）和（81．79±1．77）％（P=0．008），均显著高于OGD组的（70．62±8．89）％。1μmol／L、5μm和10μm几刺槐素组SH．SY5Y胞LDH漏出率分别为（159．11±13．11）％（P=0．021）、（155．12±24．72）％（P=0．011）和（160．92±7．83）％（P=0．027），均显著低于OGD组的（180．35±10．60）％。体内实验显示，大剂量刺槐素组神经功能评分显著低于与脑缺血再灌注组[（1．67±0．85）分对（2．50±0．55）分；P=0．018]。小剂量、中剂量和大剂量刺槐素组梗死体积分别为（24．14±7．10）mm3、（17．18±3．19）mm3和（12．86±1．88）mm3，均显著小于脑缺血再灌注组的[（48．81±9．48）mm3]（P均=0．000）。结论体外和体内实验均显示，刺槐素具有神经保护作用。
Objective To investigate the neuroprotective effects of acacetin in oxygen-glucose deprivation in SH-SYSY cells and cerebral ischemia/reperfusion in mice. Methods Neuroblastoma cells （SH-SYSY） were cultured, and they were divided into the normal group, oxygen-glucose deprivation （OGD） group, and the groups of 1, 5 and 10 μmol/L acacetin. After 24-h reoxygenation, the MTT method was used to assay the cell viability and lactate dehydrogenase （LDH） assay was used to measure the LDH release rate. A total of 60 C57 mice were randomly divided into 5 groups： the sham operation group, the ischemia-reperfusion group and the groups of low moderate- and high-dose acacetin （n = 12 in each group）. A model of middle cerebral artery occlusion （MCAO） was induced by the intraluminal suture method. During the ischemia-reperfusion period, acacetin was injected intraperitoneally （6. 25, 12. 5, and 25 mg/kg, respectively ）. The neurological function score was evaluated at 24 hours after reperfusion. Cerebral infarct volume was determined by using 2, 3,5-triphenyl tetrazolium chloride staining. Results The in vitro experiments showed that the SH-SY5Y cell survival rates in the groups of 1 μmol/L, 5 txmol/L and 10 μmol/L acacetin were 90. 34% ± 6. 87% （P = 0. 000）, 85. 47% 2. 24% （P =0. 001） and 81.79% ± 1.77% （P =0. 008）, respectively, they were significantly higher than that in the OGD group （70. 62% ± 8. 89%）. The LDH release rates in the groups of 1 izmol/L （P = 0. 021 ）, 5 txmol/L （P =0. 011） and 10 txmol/L （P = 0. 027） acacetin were 159. 11% ± 13. 11%, 155. 12% ± 24. 72% and 160. 92% ±7. 83%, respectively, they were significantly lower than that in the OGD group （180. 35% ± 10. 60%）. The in vivo experiments showed that the neurological function score in the group of high-dose acacetin was lower than that in the ischemia-reperfusion group （1.67 ±0. 85 vs. 2. 50 ±0. 55; P =0. 018）; the infarct volumes in the groups of low-, moderate-, and high-dose acacetin w
出处
《国际脑血管病杂志》
北大核心
2013年第1期47-50,共4页
International Journal of Cerebrovascular Diseases
